Determination of lutein in infant formula milk powder using ultra-high performance liquid chromatography

doi:10.3724/SP.J.1123.2013.10007

Abstract

Abstract:

An ultra-high performance liquid chromatography (U-HPLC) method for the determination of lutein in the infant formula milk powder was developed. The sample was extracted with acetone and defatted using freezing centrifugation method. The U-HPLC separation was achieved using a YMC Carotenoid C30 column (150 mm×4.6 mm, 3 μm) with the mixture of methanol/methyl tert-butyl ether (70:30, v/v) as the mobile phase under isocratic elution. The flow rate was 0.5 mL/min and the column oven temperature was 25 ℃. The injection volume was 5 μL. It was detected on a photodiode array detector at a wavelength of 445 nm. The results showed that the linear range was 20-500 μg/L (r=0.999 9), and the limit of quantification was 20 μg/L. The mean recoveries of lutein varied from 97.9% to 104.4% spiked at 50, 250 and 2000 μg/kg. The established method is simple, accurate and sensitive for the rapid determination of lutein in infant formula milk powder.

Key words: infant formula milk powder, lutein, ultra-high performance liquid chromatography (U-HPLC)

CLC Number:

O658

WANG Lina, HUANG Junrong, ZHANG Li, FENG Feng, LING Yun, CHU Xiaogang, LI Hongliang. Determination of lutein in infant formula milk powder using ultra-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2013, 31(12): 1228-1231.

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