Chinese Journal of Chromatography ›› 2016, Vol. 34 ›› Issue (3): 351-355.DOI: 10.3724/SP.J.1123.2015.11013

• Technical Notes • Previous Articles    

Determination of 1,3-dioleoyl-2-palmitoyl triglyceride in infant formula milk powder by normal-phase liquid chromatography

SONG Ge, KANG Meijuan, YANG Jinbao   

  1. National Dairy Testing Center, Northeast Agriculture University, Harbin 150028, China
  • Received:2015-11-09 Online:2016-03-08 Published:2012-09-28

Abstract:

Methods for the determination of 1,3-dioleoyl-2-palmitoyl triglyceride (OPO) in infant formula milk powder by normal-phase liquid chromatography (NP-LC) and reversed-phase liquid chromatography (RP-LC) were established and compared. The analytes in the infant formula were extracted by ethyl ether and petroleum ether, enriched by a rotary evaporator, and separated on silver-ion column and C18 column and detected with an evaporative light scattering detector (ELSD). OPO and 1,2-dioleoyl-3-palmitoyl triglyceride (OOP) can be basically separated on a silver-ion column with a mixed solution of dichloromethane-acetone as mobile phase by gradient elution. The recoveries were 93.0%-101.5%, the relative standard deviations (RSD) were 2.89%-4.56%, and the limit of detection was 20 mg/kg. This method is accurate and stable for the determination of OPO in the infant formula milk powder.

Key words: 1,3-dioleoyl-2-palmitoyl triglyceride (OPO), infant formula milk powder, normal-phase liquid chromatography (NP-LC), reversed-phase liquid chromatography (RP-LC)

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