Chinese Journal of Chromatography ›› 2014, Vol. 32 ›› Issue (4): 319-329.DOI: 10.3724/SP.J.1123.2014.01039

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Recent advances in sample preparation methods of plant hormones

WU Qian1,2, WANG Lu3, WU Dapeng1, DUAN Chunfeng1, GUAN Yafeng1,3   

  1. 1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. Department of Chemistry, University of Science & Technology of China, Hefei 230026, China
  • Received:2014-01-23 Revised:2014-03-13 Online:2014-04-08 Published:2014-03-28

Abstract:

Plant hormones are a group of naturally occurring trace substances which play a crucial role in controlling the plant development, growth and environment response. With the development of the chromatography and mass spectroscopy technique, chromatographic analytical method has become a widely used way for plant hormone analysis. Among the steps of chromatographic analysis, sample preparation is undoubtedly the most vital one. Thus, a highly selective and efficient sample preparation method is critical for accurate identification and quantification of phytohormones. For the three major kinds of plant hormones including acidic plant hormones & basic plant hormones, brassinosteroids and plant polypeptides, the sample preparation methods are reviewed in sequence especially the recently developed methods. The review includes novel methods, devices, extractive materials and derivative reagents for sample preparation of phytohormones analysis. Especially, some related works of our group are included. At last, the future developments in this field are also prospected.

Key words: brassinosteroids, gibberellins, plant hormones, plant polypeptide, sample preparation

CLC Number: