Abstract:

The chemical constituents in different tissues (primary cuticula, epidermis, peel, pulp, seed) of the three kinds of pomelo (Shatian pomelo, Guanxi honey pomelo, Yuhuan pomelo) in China were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry, liquid chromatography-electrospray ion trap-mass spectrometry and ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. The results showed that 68 different chemical constituents were identified in the five tissues of the three kinds of pomelo (19 alcohols, 15 esters, 9 olefins, 8 organic acids, 7 ketones, 4 alkanes and 6 other substances). Among which 7 common constituents were found in primary cuticula, and the highest content was 44.03%(limonene). In epidermis, the highest content in 6 common constituents was 44.62%(oleic acid amide). In 10 common constituents of peel, the highest content was 30.75%(sitosterol). The highest content was 20.02% (palmitic acid) of 11 common constituents in pulp. In seed, the highest content of 15 common constituents was 45.83%( α-linoleic acid). We also applied the cluster analysis and similarity analysis to the chemical constituents of different parts of the three kinds of pomelo. It is apparent that there are differences and similarities in species and contents of the constituents in the same parts of different varieties of pomelo. The chromatographic fingerprint specificity is obvious, which is helpful to put forward a new idea of chromatographic fingerprint in classification, quality control and geographical tracing of pomelo.

Key words: analysis of the divergences, chemical constituents, chromatography-mass spectrometry, cluster analysis, fingerprint, pomelo