Abstract:

Protein phosphorylation is one of the most important post-translational modifications in life activities, which has a close relationship to the precise regulation of life activities. Thus, preparation of materials capable of selective enrichment of phosphoproteins is significantly important to phosphoproteome research. In this research, we prepared poly(guanidinium ionic liquid)s materials by using a synthesized guanidinium ionic liquid functional monomer. Poly(guanidinium ionic liquid)s materials were characterized by Fourier transform infrared spectrometer (FT-IR), scanning electron microscope (SEM) and thermo gravimetric analyzer (TGA). The results indicated that microspheres had a mean size of 200 nm in diameter. Using β-casein as a model phosphoprotein, the recognition properties of the obtained materials for phosphoprotein were evaluated. The adsorption results showed that microspheres not only had a high adsorption capacity for phosphorylation proteins (599.1 mg/g for β-casein) and a rapid balance in 1 h, but also displayed high selectivity to phosphorylation proteins.

Key words: enrichment, guandine, ionic liquids, phosphorylation protein, preparation