Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2016, Vol. 34 ›› Issue (10): 956-959.DOI: 10.3724/SP.J.1123.2016.05018

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Simultaneous determination of sucralose, glucose, fructose and sucrose in beverage by ion-exchange chromatography with column-switching

CHEN Ning1,2, ZHANG Peimin1, WU Shuchao3, ZHU Yan1,2   

  1. 1. Department of Chemistry, Zhejiang University, Hangzhou 310028, China;
    2. Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Zhejiang University, Hangzhou 310028, China;
    3. Zhejiang Institute of Geology and Mineral Resources, Hangzhou 310007, China
  • Received:2016-05-26 Online:2016-10-08 Published:2013-04-03
  • Supported by:

    National Natural Science Foundation of China (No. 21405141); National Important Project on Science Instrument (No. 2012YQ09022903); Zhejiang Provincial Natural Science Foundation of China (Nos. LZ16B050001, LY15B050001, S16H180002); Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province (Nos. 2014006, 2014007); Open Foundation of the Key Laboratory of Clay Minerals, the Ministry of Land and Resources of China (No. 2014-K03).

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Abstract:

A new method of simultaneous determination of sucralose, glucose, fructose and sucrose in beverage has been established using ion-exchange chromatography coupled with column-switching and pulsed amperometric detection. A CarboPac PA10 guard column and a CarboPac PA10 analytical column, along with the mixtures of pure water and 250 mmol/L NaOH solution in different ratio acting as mobile phase in the gradient elution were used in the pulsed amperometric detection. After the separation and detection of saccharides with weak retention through elusion with 25 mmol/L NaOH, we switched the guard column to analytical column, in which sucralose can be separated through the comparatively short guard column while the four saccharides can be detected simultaneously. In this study, four saccharides had good linear relationships in 0.01-20 mg/L and the recoveries were 90.38%-102.88% (sucralose), 95.56%-102.75% (glucose), 101.66%-114.33% (fructose), 105.03%-106.49% (sucrose) with high sensitivity and good reproducibility. This method can be widely used in the determination of components with strong retention in food.

Key words: column-switching, ion-exchange chromatography, sucralose

CLC Number: