Chinese Journal of Chromatography ›› 2014, Vol. 32 ›› Issue (12): 1380-1384.DOI: 10.3724/SP.J.1123.2014.07052

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Determination of fructooligosaccharides in milk powder using high performance anion-exchange chromatography coupled with pulsed amperometric detection

GENG Lijuan1,2, HUANG Junrong1, FENG Feng2, JIANG Pingping2, CHU Xiaogang2, ZHANG Feng2, LING Yun2   

  1. 1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;
    2. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, China
  • Received:2014-07-31 Revised:2014-08-25 Online:2014-12-08 Published:2014-11-28

Abstract:

Fructooligosaccharides (FOS) are usually added in milk powder as a kind of prebiotic. Thus, quantitative analysis of the FOS is very important for the quality control of milk powder. In this study, a simple method for the simultaneous determination of three FOS components with degrees of polymerization (DP) 3-5 in milk powder was developed by high performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The samples were extracted with 50%(v/v) ethanol aqueous solution and defatted by an On Guard RP pretreatment column. The separation was performed on a CarboPac PA200 column by gradient elution using deionized water, 0.2 mol/L NaOH solution and 0.4 mol/L NaAc solution as the mobile phases. The flow rate was 0.4 mL/min. The column temperature was 30 ℃; and the injection volume was 25 μL. Good linear response was observed in the concentration range of 0.05-10 mg/L (r2>0.9993). The limits of quantification were 0.02, 0.005 and 0.02 mg/L for 1-kestose, nystose and fructofuranosyl-nystose, respectively. The mean recoveries varied from 86.0% to 114.0% at three spiked levels of 0.5, 1.0 and 5.0 mg/L. The short-chain fructooligosaccharides from inulooligosaccharides (IOS) were successfully separated by the developed HPAEC-PAD method. The method is simple, accurate, sensitive, and helpful for the quality control of milk powder.

Key words: fructooligosaccharides (FOS), high performance anion-exchange chromatography (HPAEC), milk powder, pulsed amperometric detection (PAD)

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