Determination of β-glycerophosphate and L-malate in glycerol phosphate preparations by ion chromatography

doi:10.3724/SP.J.1123.2016.05020

Abstract

Abstract:

An analytical method for the determination of β-glycerophosphate and L-malate in glycerol phosphate preparations was established by ion chromatography with isocratic elution. α -Glycerol phosphate was completely separated from β-glycerol phosphate in the glycerol phosphate preparations. A Metrosep A Supp7 column (250 mm×4.0 mm, 5 μm) was used for separation with the eluent of 3.6 mmol/L Na₂CO₃ at a flow rate of 0.7 mL/min. The column temperature was 35℃. With this method, good accuracy and precision were obtained. The recoveries of the method ranged from 90.73% to 106.27%. The method is simple, rapid, sensitive, and suitable for the determination of β-glycerophosphate and L-malate in glycerol phosphate preparations.

Key words: β-glycerophosphate, glycerol phosphate preparations, ion chromatography (IC), L-malate

CLC Number:

O658

LIU Yuxiu, YU Hongwei. Determination of β-glycerophosphate and L-malate in glycerol phosphate preparations by ion chromatography[J]. Chinese Journal of Chromatography, 2016, 34(10): 964-967.

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