Determination of seven monosaccharides in aloe polysaccharide using ion chromatography with pulsed amperometric detector

doi:10.3724/SP.J.1123.2018.11019

Abstract

Abstract:

Aloe polysaccharide was extracted by water extraction and ethanol precipitation. It was hydrolyzed by trifluoroacetic acid, eluted by a sodium hydroxide gradient on a Dionex CarboPac PA10 column, and detected using a pulsed amperometric detector. This method was used to determine the fucose, rhamnose, arabinose, galactose, glucose, mannose, and xylose in aloe polysaccharide. The results showed that the correlation coefficients (R²) of all the seven target compounds were more than 0.997 in the linear range, and the limits of detection were between 0.007 mg/L and 0.024 mg/L. The recoveries were in the range 97.5%-102.5% with the relative standard deviations in the range 0.1%-4.8%. The method is simple, rapid, sensitive, and accurate for the determination of the monosaccharide content and the constitution of aloe polysaccharides.

Key words: aloe polysaccharide, ion chromatography (IC), monosaccharide, pulsed amperometric detector (PAD)

CLC Number:

O658

LE Shengfeng, WANG Wei, WANG Yaxuan, ZHAO Xinying. Determination of seven monosaccharides in aloe polysaccharide using ion chromatography with pulsed amperometric detector[J]. Chinese Journal of Chromatography, 2019, 37(3): 319-324.

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