Chinese Journal of Chromatography ›› 2016, Vol. 34 ›› Issue (12): 1199-1206.DOI: 10.3724/SP.J.1123.2016.08047

• Reviews • Previous Articles     Next Articles

Recent advances in separation methods for post-translational modification proteomics

XU Jingjing1,2, LIU Xing1, ZHOU Hu1   

  1. 1. Department of Analytical Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
    2. Chemical Drugs Department, Shanghai Institute of Food and Drug Control, Shanghai 201203, China
  • Received:2016-08-31 Online:2016-12-08 Published:2013-04-24
  • Supported by:

    National Natural Science Foundation of China (No. 21375138); "One Hundred Talented People" of the Chinese Academy of Sciences; Youth Science Fund Project of National Natural Science Foundation of China (No. 31500110); Jiangsu Province Key Laboratory of Children's Respiratory Diseases (Traditional Chinese Medicine) Open Research Topic (No. JKLPRD201404).

Abstract:

Post-translational modifications (PTMs) are the key control of many cellular processes. Current progresses of separation methods for PTMs proteomics, including reversed phase (RP) chromatography, ion exchange (IEX) chromatography, hydrophilic interaction chromatography (HILIC), porous graphitic carbon (PGC) chromatography, capillary electrophoresis (CE) and size-exclusion chromatography (SEC) are reviewed. The methods for the identification of PTMs have higher resolution and sensitivity. In addition, some other important separation methods for proteomics study are introduced, and they may be applied in the study of PTMs with further optimization in the future.

Key words: capillary electrophoresis (CE), hydrophilic interaction chromatography (HILIC), ion exchange (IEX) chromatography, porous graphitic carbon (PGC) chromatography, post-translational modifications (PTMs), proteomics, reversed phase (RP) chromatography

CLC Number: