Synthesis of AAPBA@KCC-1 and its application in the analysis of sialic acid and gangliosides in serum

doi:10.3724/SP.J.1123.2017.01025

Abstract

Abstract:

A new material AAPBA@KCC-1 was synthesized by using 3-acrylamidophenylboronic acid (AAPBA) modified on mesoporous silica KCC-1. First, AAPBA was modified on the surface of KCC-1 by three-step synthesis method. Then, AAPBA@KCC-1 was characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), N₂ adsorption-desorption isotherm and X-ray photoelectron spectroscopy (XPS). AAPBA@KCC-1 was used as the dispersive solid-phase extraction (dSPE) adsorbent to extract and enrich sialic acid and ganglioside (GLS) from serum samples. Finally, Fourier transform ion cyclotron resonance mass spectrometry (FT-MS) and ultra performance liquid chromatography-ion mobility spectrometry-quadrupole time of flight mass spectrometry (UPLC-IMS-QTOF MS) were used to evaluated the adsorption ability of AAPBA@KCC-1 to sialic acid and GLS, respectively. The results showed that AAPBA@KCC-1 can effectively adsorb sialic acid and GLS, and can be used for further studies of sialic acid and GLS in biological samples.

Key words: N-acetylneuraminic acid (Neu5Ac), 3-acrylamidophenylboronic acid (AAPBA), dispersive solid-phase extraction (dSPE), ganglioside (GLS), mesoporous silica KCC-1, sialic acid

CLC Number:

O658

TIAN Huajun, XUE Yun, CHEN Qiaomei, WANG Yan, YAN Chao. Synthesis of AAPBA@KCC-1 and its application in the analysis of sialic acid and gangliosides in serum[J]. Chinese Journal of Chromatography, 2017, 35(5): 473-481.

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