Preparation of zirconia nanoparticles deposited cotton fibers and their application in the rapid enrichment of phosphopeptides

doi:10.3724/SP.J.1123.2017.11055

Abstract

Abstract:

A kind of cotton-based fibrous adsorbent, zirconia nanoparticles coated cotton fiber (ZrO₂-CF), was successfully prepared by liquid-phase deposition of zirconia nanoparticles on the surface of cotton fiber. And the ZrO₂-CF fibrous adsorbent was packed in pipet tips for the enrichment of phosphopeptide by pipetting up and down within 2 min, which was simple and fast. Nine phosphopeptides were identified from the digestion of β-casein after ZrO₂-CF extraction. ZrO₂-CF exhibited good selectivity in the enrichment of phosphopeptides from the digestion mixture of β-casein and BSA (molar ratio is 1:100) in which four phosphopeptides were identified. And it also showed excellent extraction efficiency in actual biological samples such as non-fat milk and human serum in which four and nine phosphopeptides were identified, respectively.

Key words: enrichment, liquid phase deposition, nanoparticles, phosphopeptide, zirconia

CLC Number:

O658

YU Qiongwei, FANG Kaimin, HE Xiaomei, ZHENG Jie, FENG Yuqi. Preparation of zirconia nanoparticles deposited cotton fibers and their application in the rapid enrichment of phosphopeptides[J]. Chinese Journal of Chromatography, 2018, 36(3): 237-244.

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