Chinese Journal of Chromatography ›› 2022, Vol. 40 ›› Issue (8): 694-703.DOI: 10.3724/SP.J.1123.2022.03002
• Special Columnof Separation and Analysis for Food Safety-Functional Adsorbents • Previous Articles Next Articles
LI Xiaohan1, LU Yingying1, DONG Yongzhen1, JIANG Feng2, FAN Zhiyong2, PAN Hui3, LIU Mingjun3, CHEN Yiping1,*()
Received:
2022-03-03
Online:
2022-08-08
Published:
2022-07-29
Contact:
CHEN Yiping
Supported by:
CLC Number:
LI Xiaohan, LU Yingying, DONG Yongzhen, JIANG Feng, FAN Zhiyong, PAN Hui, LIU Mingjun, CHEN Yiping. Separation and enrichment of trace aflatoxin B1 in grains by magnetic nanomaterials based on SiO2@Fe3O4[J]. Chinese Journal of Chromatography, 2022, 40(8): 694-703.
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URL: https://www.chrom-china.com/EN/10.3724/SP.J.1123.2022.03002
Fig. 3 Effects of (a) the ratio of the amount of EDC to NHS, (b) pH, concentration of MES and (c) coupling time on antibody coupling efficiency (n=3) EDC: 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; NHS: N-hydroxysuccinimide substances; MES: 2-morpholinoethanesulfonic acid monohydrate.
Fig. 4 (a) Transmission electron microscopy (TEM), (b) dynamic light scattering (DLS) and (c) fourier transform-infrared spectroscopy (FT-IR) characterizations of SiO2@Fe3O4
Fig. 6 (a) Enrichment capacities of Ab-SiO2@Fe3O4 and Ab-Fe3O4 and (b) specificities of Ab-SiO2@Fe3O4 materials (n=3) OTA: ochratoxin A; DON: deoxynivalenol; ZEN: zearalenone.
Repeat time | Spiked/ (μg/kg) | Detected/ (μg/kg) | Recovery/ % | RSD (n=5)/ % |
---|---|---|---|---|
1 | 0.1 | 0.086 | 85.86 | 4.36 |
0.2 | 0.165 | 82.73 | 4.07 | |
0.5 | 0.446 | 89.26 | 3.95 | |
2 | 0.1 | 0.094 | 83.95 | 2.98 |
0.2 | 0.166 | 82.92 | 3.01 | |
0.5 | 0.444 | 88.73 | 3.62 | |
3 | 0.1 | 0.087 | 86.82 | 4.18 |
0.2 | 0.163 | 81.51 | 4.29 | |
0.5 | 0.450 | 89.96 | 4.32 | |
4 | 0.1 | 0.085 | 84.57 | 3.15 |
0.2 | 0.163 | 81.34 | 3.67 | |
0.5 | 0.448 | 89.61 | 4.96 | |
5 | 0.1 | 0.085 | 84.61 | 3.75 |
0.2 | 0.161 | 80.58 | 2.84 | |
0.5 | 0.4382 | 87.64 | 3.08 |
Table 1 Recoveries and reusabilities of Ab-SiO2@Fe3O4 magnetic nanomaterials in wheat samples
Repeat time | Spiked/ (μg/kg) | Detected/ (μg/kg) | Recovery/ % | RSD (n=5)/ % |
---|---|---|---|---|
1 | 0.1 | 0.086 | 85.86 | 4.36 |
0.2 | 0.165 | 82.73 | 4.07 | |
0.5 | 0.446 | 89.26 | 3.95 | |
2 | 0.1 | 0.094 | 83.95 | 2.98 |
0.2 | 0.166 | 82.92 | 3.01 | |
0.5 | 0.444 | 88.73 | 3.62 | |
3 | 0.1 | 0.087 | 86.82 | 4.18 |
0.2 | 0.163 | 81.51 | 4.29 | |
0.5 | 0.450 | 89.96 | 4.32 | |
4 | 0.1 | 0.085 | 84.57 | 3.15 |
0.2 | 0.163 | 81.34 | 3.67 | |
0.5 | 0.448 | 89.61 | 4.96 | |
5 | 0.1 | 0.085 | 84.61 | 3.75 |
0.2 | 0.161 | 80.58 | 2.84 | |
0.5 | 0.4382 | 87.64 | 3.08 |
Food matrix | Spiked/ (μg/kg) | Detected/ (μg/kg) | Recovery/ % | RSD/% |
---|---|---|---|---|
Rice | 0.1 | 0.089 | 88.75 | 4.88 |
0.2 | 0.164 | 82.06 | 2.86 | |
0.5 | 0.393 | 78.57 | 3.16 | |
1.0 | 0.892 | 89.15 | 5.19 | |
Corn | 0.1 | 0.083 | 83.25 | 4.55 |
0.2 | 0.152 | 76.21 | 3.28 | |
0.5 | 0.434 | 86.71 | 2.81 | |
1.0 | 0.818 | 79.45 | 4.94 | |
Wheat | 0.1 | 0.093 | 92.85 | 3.69 |
0.2 | 0.172 | 86.03 | 5.29 | |
0.5 | 0.454 | 90.87 | 4.21 | |
1.0 | 0.886 | 88.56 | 3.95 |
Table 2 Spike recoveries of AFB1 at four levels in three grain matrices (n=5)
Food matrix | Spiked/ (μg/kg) | Detected/ (μg/kg) | Recovery/ % | RSD/% |
---|---|---|---|---|
Rice | 0.1 | 0.089 | 88.75 | 4.88 |
0.2 | 0.164 | 82.06 | 2.86 | |
0.5 | 0.393 | 78.57 | 3.16 | |
1.0 | 0.892 | 89.15 | 5.19 | |
Corn | 0.1 | 0.083 | 83.25 | 4.55 |
0.2 | 0.152 | 76.21 | 3.28 | |
0.5 | 0.434 | 86.71 | 2.81 | |
1.0 | 0.818 | 79.45 | 4.94 | |
Wheat | 0.1 | 0.093 | 92.85 | 3.69 |
0.2 | 0.172 | 86.03 | 5.29 | |
0.5 | 0.454 | 90.87 | 4.21 | |
1.0 | 0.886 | 88.56 | 3.95 |
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