Determination of maleic hydrazide and its glucosides in tobacco leaves using hydrophilic interaction liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2018.01050

Abstract

Abstract:

A hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method was established for the simultaneous determination of maleic hydrazide (MH) and its two glucosides in tobacco leaves. Ultrasonic assisted extraction of MH and its glucosides was performed using acetonitrile-methyl tert-butyl ether-water (7:10:13, volume ratio). The extraction solution was then centrifuged, and the subnatant was transferred for solvent replacement using acetonitrile. The extract in acetonitrile was then analyzed using HILIC-MS/MS. Method validation was performed, and the linear ranges for MH and MH-O-β -D-glucoside were 5-150 mg/kg with correlation coefficients (r²) greater than 0.9971 for matrix-matched calibration curves. Limits of detection for MH and MH-O-β -D-glucoside were 0.5 mg/kg and 0.3 mg/kg, and limits of quantification were 1.0 mg/kg and 0.8 mg/kg, respectively. The recoveries were in the range of 83.1%-112.3% at three spiked levels (10, 40, 80 mg/kg), with intra-day repeatability of 2.7% and 3.8%, inter-day repeatability of 8.3% and 7.1% at 40 mg/kg. The established method was used for the study of MH metabolism in tobacco leaves. By the 28th day after MH spraying, the content of MH in tobacco leaves had decreased by 80.8%, of which only 7.6% transformed to MH-glucosides. The disposition of the remainder needs to be studied.

Key words: glucoside, hydrophilic interaction liquid chromatography(HILIC), maleic hydrazide, tandem mass spectrometry(MS/MS), tobacco leaves

CLC Number:

O658

PANG Tao, LIN Qian, LI Yong, SHI Junli, DENG Xiaopeng, KONG Guanghui, LU Xiuping, JIN Yan. Determination of maleic hydrazide and its glucosides in tobacco leaves using hydrophilic interaction liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2018, 36(7): 651-658.

References

[1] Schoene D L, Hoffmann O L. Science, 1949, 109(2841):588
[2] Barnes J M, Magee P N, Boyland E, et al. Nature, 1957, 180(4576):62
[3] Mannell W A, Grice H C. Can J Biochem Physiol, 1957, 35(12):1233
[4] Swietlińska Z, Zuk J. Mutat Res, 1978, 55(1):15
[5] Codex Alimentarius Pesticides Database Search.[2018-01-29]. http://www.fao.org/fao-who-codexalimentarius/codex-texts/dbs/pestres/pesticide-detail/en/?p_id=102
[6] CORESTA Guide No 1. The Concept and Implementation of CPA Guidance Residue Levels.[2018-01-29]. https://www.coresta.org/sites/default/files/technical_documents/main/Guide-No01-GRLs4th-Issue-July16. pdf
[7] YQ50-2014. The Maximun Residue Level of Pesticides in Tobacco Leaves. Enterprise Standard of China National Tobacco Corporation, 2014 YQ50-2014. 烟叶农药最大残留量. 中国烟草总公司企业标准, 2014
[8] Frear D S, Swanson H R. J Agric Food Chem, 1978, 26:660
[9] Tagawa H, Tobita T, Tomita H, et al. Biosci Biotech Biochem, 1995, 59(9):1753
[10] Chen X S, Bian Z Y, Yang F, et al. Chinese Journal of Chromatography, 2013, 31(11):1116 陈晓水, 边照阳, 杨飞, 等. 色谱, 2013, 31(11):1116
[11] Si X X, Lu S M, Liu Z H, et al. Chinese Journal of Chromatography, 2016, 34(3):340 司晓喜, 陆舍铭, 刘志华, 等. 色谱, 2016, 34(3):340
[12] Si X X, Zhu R Z, Zhang F M, et al. Chinese Journal of Chromatography, 2016, 34(6):608 司晓喜, 朱瑞芝, 张凤梅, 等. 色谱, 2016, 34(6):608
[13] Mamani M C, Stadler T, da Silva A A, et al. Talanta, 2012, 89:369
[14] Chen X S, Zhu S X, Jiang J L, et al. Journal of Chinese Mass Spectrometry Society, 2017, 38(2):234 陈晓水, 朱书秀, 蒋佳磊, 等. 质谱学报, 2017, 38(2):234
[15] Wang L, Fei T, Qi D, et al. Anal Methods, 2015, 7:5103
[16] Newsome W H. J Agric Food Chem, 1980, 28(2):270
[17] Gong C R, Zhou Y H, Yang H W. Introduction for Three Stages Curing of Flue-Cured Tobacco. Beijing:Science Press, 2006 宫长荣, 周义和, 杨焕文. 烤烟三段式烘烤导论. 北京:科学出版社, 2006
[18] Dattilo B S, Gallo S, Lionetti G, et al. Beitriige zur Tabakforschung Int, 1994, 16(2):57
[19] Paya P, Anastassiades M, Mack D, et al. Anal Bioanal Chem, 2007, 389(6):1697
[20] Mamani M C, Stadler T, da Silva A A, Barbosa L C A, et al. Talanta, 2012, 89:369
[21] Towers G H N, Hutchinson A. Nature, 1958, 181:1535
[22] Guidance Document on Analytical Quality Control and Method Validation Procedures for Pesticides Residues Analysis in Food and Feed.[2018-01-29]. https://ec.europa.eu/food/sites/food/files/plant/docs/pesticides_mrl_guidelines _wrkdoc_2017-11813.pdf