Qualitative identification of impurities and principal component quantitative analysis of zearalanone on purity certified reference material

doi:10.3724/SP.J.1123.2019.04028

Abstract

Abstract: A liquid chromatography method was established for the determination of zearalanone (ZAN) raw material. The qualitative analysis of ZAN and its trace impurities was performed by ultra performance liquid chromatography-diode array detector (UPLC-DAD) and ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS), and the response factors of each impurity were calculated. The three main organic impurities in the ZAN raw material were identified as β -zearalanol, α -zearalanol and a dehydration product of zearalanol with relative response factors of 0.5352, 0.8594 and 0.6973, respectively. The main component of the ZAN raw material was determined by the calibration factor normalization method. The purity of zearalanone was determined to be 99.6% with a standard deviation of 0.01%. This method can provide a technical support for the development of ZAN standard materials.

Key words: certified reference material, high resolution mass spectrometry (HRMS), impurity identification, ultra performance liquid chromatography-diode array detection (UPLC-DAD), zearalanone (ZAN)

CLC Number:

O658

FENG Mengyu, LIU Shuan, GAO Yan, ZHOU Shukun, GUO Zhen, LI Xiuqin, ZHANG Qinghe. Qualitative identification of impurities and principal component quantitative analysis of zearalanone on purity certified reference material[J]. Chinese Journal of Chromatography, 2019, 37(8): 911-917.

References

[1] Zhang L X, Sun J Y, Wang Y H, et al. Chinese Journal of Chromatography, 2018, 36(6):566章璐幸, 孙洁胤, 王延辉, 等. 色谱, 2018, 36(6):566
[2] Jing P, Zhang Y J, Liang Q C, et al. Food Research and Development, 2018, 34(23):72静平, 张幼筠, 梁启超, 等. 食品研究与开发, 2018, 34(23):72
[3] GB/T 21982-2008
[4] GB/T 22992-2008
[5] GB/T 22963-2008
[6] GB/T 20766-2006
[7] You L N.[MS Dissertation]. Chongqing:Chongqing Medical University, 2013游丽娜.[硕士学位论文]. 重庆:重庆医科大学, 2013
[8] Kinani S, Bouchonnet S, Bourcier S, et al. J Chromatogr A, 2008, 1190:307

[9] Lai S Y, Tao B H, Fu S S, et al. Chinese Journal of Analytical Chemistry, 2012, 40(1):135赖世云, 陶保华, 傅士姗, 等. 分析化学, 2012, 40(1):135
[10] Cui X Y, Zhang X F, Li Z, et al. China Dairy Industry, 2015, 43(10):44崔向云, 张雪峰, 李照, 等. 中国乳品工业, 2015, 43(10):44
[11] Krska R, Welzig E, Josrphs R D. J AOAC Int, 2003, 86(4):722
[12] Zhong S H, Ye J M, Ye L H, et al. Farm Products Processing, 2018, 12:59钟世欢, 叶佳明, 叶磊海, 等. 农产品加工, 2018, 12:59
[13] Li C L, Deng C L, Zhou S, et al. Anal Bioanal Chem, 2018, 410(21):5301

[14] Qian M R, Zhang H, Wu L Q, et al. Food Chem, 2015, 166:23

[15] Zhang X Y, Tang Q Q, Mi T J, et al. Food Control, 2018, 87:100

[16] Meng J, Zhang J, Zhang N, et al. Chinese Journal of Chromatography, 2010, 28(6):601孟娟, 张晶, 张楠, 等. 色谱, 2010, 28(6):601

[17] Pamel E V, Vlaemynck G, Heyndrickx M, et al. Mycotox Res, 2011, 27:37

[18] Brezina U, Valenta H, Rempe I, et al. Mycotox Res, 2014, 30:171

[19] Bracher F, Krauss J. Monatshefte Fur Chemie, 2001, 132(7):805

[20] Qian Z Z, Liu Z Y, Deng W J, South China Fisheries Science, 2011, 7(1):62钱卓真, 刘智禹, 邓武剑, 等. 南方水产科学, 2011, 7(1):62
[21] Pharmacopoeia Commission of the People's Republic of China. Pharmacopoeia of the People's Republic of China, Part 4. Beijing:China Medical Science Press, 2015:61国家药典委员会. 中华人民共和国药典, 四部. 北京:中国医药科技出版社, 2015:61
[22] Zhao J D, Zhang H Z, Wen H L, et al., Drug Standards of China, 2018, 19(5):382赵敬丹, 张含智, 闻宏亮, 等. 中国药品标准, 2018, 19(5):382
[23] Liu G M. Drug Standards of China, 2018, 19(5):353刘光敏. 中国药品标准, 2018, 19(5):353
[24] Hou X F, Shi Y D, Wang S C. Journal of China Pharmaceutical University, 2019, 50(1):33侯晓芳, 施迎娣, 王嗣岑. 中国药科大学学报, 2019, 50(1):33
[25] Li S, Luo L, Zhang W F, et al. Journal of Shenyang Pharmaceutical University, 2019, 36(1):39李帅, 罗林, 张卫锋, 等. 沈阳药科大学学报, 2019, 36(1):39
[26] Yu Z X, Yu L J, Huang H W. Drug Standards of China, 2011, 11(4):278余振喜, 瘐莉菊, 黄海伟. 中国药品标准, 2011, 11(4):278
[27] Zuo W F, Pan N, Fan X P. China Pharmacy Journal, 2012, 47(24):2029左文飞, 潘娜, 范雪平. 中国药学杂志, 2012, 47(24):2029