Chinese Journal of Chromatography ›› 2023, Vol. 41 ›› Issue (10): 921-928.DOI: 10.3724/SP.J.1123.2023.02011
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NIE Yangyang1, YANG Guantao2, WANG Haiyan1, QIAO Xiaoqiang1,*()
Received:
2023-02-24
Online:
2023-10-08
Published:
2023-10-23
Supported by:
CLC Number:
NIE Yangyang, YANG Guantao, WANG Haiyan, QIAO Xiaoqiang. Modified styrene-maleic anhydride copolymer-based chromatographic stationary phase for phospholipid separation and analysis[J]. Chinese Journal of Chromatography, 2023, 41(10): 921-928.
Fig. 1 Schematic diagram for preparation of Sil-SMA-MME stationary phase MPS: 3-mercaptopropyltriethoxysilane; DMF: N,N-dimethylformamide; TsOH: p-toluenesulfonic acid.
Fig. 3 Effect of ACN volume fractions in mobile phase on chromatographic retention for amide compounds Chromatographic conditions: mobile phase, ACN-H2O; flow rate, 1.0 mL/min; detection wavelength, 214 nm.
Fig. 4 Separation chromatograms of amides on (a) the Sil-SMA-MME column and (b, c) commercial amino column (SiO2-NH2) Chromatographic conditions: mobile phase, ACN-H2O (96∶4, v/v) for a and b, 100% ACN for c; UV detection wavelength, 214 nm; flow rate, 1.0 mL/min.Analytes: 1. cyanoacetamide; 2. 2-iodoacetamide; 3. benzamide; 4. p-aminobenzamide; 5. nicotinamide.
Fig. 5 Separation chromatograms of nucleosides and nucleic acid bases on (a) the Sil-SMA-MME column and (b, c) SiO2-NH2 column Chromatographic conditions: mobile phase, ACN-H2O (94∶6, v/v) for a and b, ACN-H2O (60∶40, v/v) for c; UV detection wavelength, 214 nm; flow rate, 1.0 mL/min.Analytes: 1. uracil; 2. uridine; 3. inosine.
Fig. 6 Separation chromatograms of phenols on (a) the Sil-SMA-MME column and (b, c) SiO2-NH2 column Chromatographic conditions: mobile phase, ACN-H2O (40∶60, v/v) for a and b, ACN-H2O (97∶3, v/v) for c; UV detection wavelength, 214 nm; flow rate, 1.0 mL/min.Analytes: 1. acetaminophen; 2. p-cresol; 3. p-chlorophenol; 4. 4-tert-octylphenol.
Fig. 7 Separation chromatograms of (a) different phospholipid classes and (b) different PC molecules on the Sil-SMA-MME column Chromatographic conditions: mobile phase for a, A-B (80∶20, v/v)(A: n-hexane-isopropanol-H2O-glacial acetic acid-triethylamine (5∶81∶14∶1.5∶0.08, v/v/v/v/v); B: MeOH); mobile phase for b, A-B (25∶75, v/v)(A: H2O; B: MeOH-ACN (8∶1, v/v)); flow rate, 1.0 mL/min; evaporative light-scattering detector.Analytes for a: 1. dipalmityl phosphatidyl serine sodium (DPPS); 2. dipalmityl phosphatidyl ethanolamine (DPPE); 3. diolyl phosphatidyl choline (DOPC). Analytes for b: 1. lysoph-osphatidylcholine (LysoPC); 2. dimyristoyl phosphatidyl choline (DMPC); 3. distearyl phosphatidyl choline (DSPC); 4. dipalmitoyl phosphatidyl choline (DPPC).
Fig. 8 Separation chromatograms of (a) phospholipid extracts from Antarctic krill oil and (b) human serum phospholipid extracts on the Sil-SMA-MME column Chromatographic conditions: mobile phase, A-B (25∶75, v/v)(A: H2O; B: MeOH-ACN (8∶1, v/v)); flow rate, 1.0 mL/min; evaporative light-scattering detector.
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