Chinese Journal of Chromatography ›› 2023, Vol. 41 ›› Issue (10): 929-936.DOI: 10.3724/SP.J.1123.2023.05008
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ZHENG Kangni, QIN Gaizhao, JIANG Xuefei, ZHANG Junhui, YUAN Liming*()
Received:
2023-05-19
Online:
2023-10-08
Published:
2023-10-23
Supported by:
CLC Number:
ZHENG Kangni, QIN Gaizhao, JIANG Xuefei, ZHANG Junhui, YUAN Liming. Preparation of porous organic cage and its use as chiral stationary phase for capillary electrochromatography[J]. Chinese Journal of Chromatography, 2023, 41(10): 929-936.
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URL: https://www.chrom-china.com/EN/10.3724/SP.J.1123.2023.05008
Sample | U/ kV | tR1/ min | tR2/ min | N1/ (plates/m) | N2/ (plates/m) | α | Rs |
---|---|---|---|---|---|---|---|
Ofloxacin | 13 | 31.78 | 31.05 | 46713 | 8806 | 1.05 | 0.56 |
15 | 20.74 | 21.61 | 42074 | 86352 | 1.06 | 1.80 | |
17 | 20.12 | 20.45 | 11081 | 9864 | 1.04 | 0.29 | |
Troger’s | 13 | 29.68 | 32.52 | 43227 | 29716 | 1.05 | 3.09 |
base | 15 | 17.86 | 19.78 | 52075 | 23254 | 1.10 | 3.33 |
17 | 17.00 | 18.27 | 32050 | 8162 | 1.07 | 1.54 | |
2-Amino- | 13 | 16.75 | 17.30 | 87567 | 45771 | 1.05 | 1.45 |
1-butanol | 15 | 13.03 | 13.42 | 103876 | 96842 | 1.04 | 1.69 |
17 | 12.45 | 13.02 | 35389 | 42168 | 1.04 | 1.60 | |
1-Phenyl- | 15 | 18.64 | 18.93 | 102065 | 39015 | 1.05 | 0.69 |
1-pentanol | 17 | 16.82 | 17.31 | 113126 | 29957 | 1.05 | 1.18 |
19 | 10.41 | 10.76 | 38061 | 10562 | 1.05 | 0.79 |
Table 1 Separation results of the four racemes at different separation voltages
Sample | U/ kV | tR1/ min | tR2/ min | N1/ (plates/m) | N2/ (plates/m) | α | Rs |
---|---|---|---|---|---|---|---|
Ofloxacin | 13 | 31.78 | 31.05 | 46713 | 8806 | 1.05 | 0.56 |
15 | 20.74 | 21.61 | 42074 | 86352 | 1.06 | 1.80 | |
17 | 20.12 | 20.45 | 11081 | 9864 | 1.04 | 0.29 | |
Troger’s | 13 | 29.68 | 32.52 | 43227 | 29716 | 1.05 | 3.09 |
base | 15 | 17.86 | 19.78 | 52075 | 23254 | 1.10 | 3.33 |
17 | 17.00 | 18.27 | 32050 | 8162 | 1.07 | 1.54 | |
2-Amino- | 13 | 16.75 | 17.30 | 87567 | 45771 | 1.05 | 1.45 |
1-butanol | 15 | 13.03 | 13.42 | 103876 | 96842 | 1.04 | 1.69 |
17 | 12.45 | 13.02 | 35389 | 42168 | 1.04 | 1.60 | |
1-Phenyl- | 15 | 18.64 | 18.93 | 102065 | 39015 | 1.05 | 0.69 |
1-pentanol | 17 | 16.82 | 17.31 | 113126 | 29957 | 1.05 | 1.18 |
19 | 10.41 | 10.76 | 38061 | 10562 | 1.05 | 0.79 |
Sample | c/ (mmol/ L) | tR1/ min | tR2/ min | N1/ (plates/ m) | N2/ (plates/ m) | α | Rs |
---|---|---|---|---|---|---|---|
Ofloxacin | 50 | 19.62 | - | 8370 | - | - | - |
100 | 20.74 | 21.61 | 42074 | 86352 | 1.06 | 1.80 | |
150 | 24.90 | 25.53 | 12740 | 30143 | 1.04 | 0.62 | |
Troger’s | 50 | 15.00 | - | 32400 | - | - | - |
base | 100 | 17.86 | 19.78 | 52075 | 23254 | 1.10 | 3.33 |
150 | 23.36 | 25.14 | 74495 | 25023 | 1.07 | 2.70 | |
2-Amino- | 50 | 11.96 | - | 217670 | - | - | - |
1-butanol | 100 | 13.03 | 13.42 | 103876 | 96842 | 1.04 | 1.69 |
150 | 14.67 | - | 495646 | - | - | - | |
1-Phenyl- | 50 | 10.48 | 10.69 | 49293 | 19822 | 1.05 | 0.62 |
1-pentanol | 100 | 16.82 | 17.31 | 113126 | 29957 | 1.05 | 1.18 |
150 | 17.99 | 18.47 | 71208 | 33356 | 1.05 | 1.03 |
Table 2 Separation results of the four racemes at different concentrations of buffer solution
Sample | c/ (mmol/ L) | tR1/ min | tR2/ min | N1/ (plates/ m) | N2/ (plates/ m) | α | Rs |
---|---|---|---|---|---|---|---|
Ofloxacin | 50 | 19.62 | - | 8370 | - | - | - |
100 | 20.74 | 21.61 | 42074 | 86352 | 1.06 | 1.80 | |
150 | 24.90 | 25.53 | 12740 | 30143 | 1.04 | 0.62 | |
Troger’s | 50 | 15.00 | - | 32400 | - | - | - |
base | 100 | 17.86 | 19.78 | 52075 | 23254 | 1.10 | 3.33 |
150 | 23.36 | 25.14 | 74495 | 25023 | 1.07 | 2.70 | |
2-Amino- | 50 | 11.96 | - | 217670 | - | - | - |
1-butanol | 100 | 13.03 | 13.42 | 103876 | 96842 | 1.04 | 1.69 |
150 | 14.67 | - | 495646 | - | - | - | |
1-Phenyl- | 50 | 10.48 | 10.69 | 49293 | 19822 | 1.05 | 0.62 |
1-pentanol | 100 | 16.82 | 17.31 | 113126 | 29957 | 1.05 | 1.18 |
150 | 17.99 | 18.47 | 71208 | 33356 | 1.05 | 1.03 |
Sample | pH | tR1/ min | tR2/ min | N1/ (plates/m) | N2/ (plates/m) | α | Rs |
---|---|---|---|---|---|---|---|
Ofloxacin | 6.5 | 27.17 | 28.42 | 8535 | 37338 | 1.05 | 1.02 |
7.5 | 20.74 | 21.61 | 42074 | 86352 | 1.06 | 1.80 | |
8.5 | 17.82 | 18.71 | 4066 | 6320 | 1.04 | 0.62 | |
Troger’s | 6.5 | 25.31 | 27.53 | 38812 | 20346 | 1.05 | 2.49 |
base | 7.5 | 17.86 | 19.78 | 52075 | 23254 | 1.10 | 3.33 |
8.5 | 14.70 | - | 16817 | - | - | - | |
2-Amino- | 6.5 | 13.85 | 14.42 | 40628 | 55390 | 1.05 | 1.60 |
1-butanol | 7.5 | 13.03 | 13.42 | 103876 | 96842 | 1.04 | 1.69 |
8.5 | 12.15 | - | 84990 | - | - | - | |
1-Phenyl- | 6.5 | 17.45 | 17.87 | 89529 | 45975 | 1.05 | 1.06 |
1-pentanol | 7.5 | 16.82 | 17.31 | 113126 | 29957 | 1.05 | 1.18 |
8.5 | 10.43 | 10.58 | 62625 | 14623 | 1.05 | 0.43 |
Table 3 Separation results of the four racemes at different pH values of buffer solution
Sample | pH | tR1/ min | tR2/ min | N1/ (plates/m) | N2/ (plates/m) | α | Rs |
---|---|---|---|---|---|---|---|
Ofloxacin | 6.5 | 27.17 | 28.42 | 8535 | 37338 | 1.05 | 1.02 |
7.5 | 20.74 | 21.61 | 42074 | 86352 | 1.06 | 1.80 | |
8.5 | 17.82 | 18.71 | 4066 | 6320 | 1.04 | 0.62 | |
Troger’s | 6.5 | 25.31 | 27.53 | 38812 | 20346 | 1.05 | 2.49 |
base | 7.5 | 17.86 | 19.78 | 52075 | 23254 | 1.10 | 3.33 |
8.5 | 14.70 | - | 16817 | - | - | - | |
2-Amino- | 6.5 | 13.85 | 14.42 | 40628 | 55390 | 1.05 | 1.60 |
1-butanol | 7.5 | 13.03 | 13.42 | 103876 | 96842 | 1.04 | 1.69 |
8.5 | 12.15 | - | 84990 | - | - | - | |
1-Phenyl- | 6.5 | 17.45 | 17.87 | 89529 | 45975 | 1.05 | 1.06 |
1-pentanol | 7.5 | 16.82 | 17.31 | 113126 | 29957 | 1.05 | 1.18 |
8.5 | 10.43 | 10.58 | 62625 | 14623 | 1.05 | 0.43 |
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