Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Separation and Identification of Chlorogenic Acid and Related Impurities by High Performance Liquid Chromatography-Tandem Mass Spectrometry

TIAN Chenxu1, XU Xiaoping1, LIAO Liyun2, ZHANG Jie1, LIU Jing1, ZHOU Sha1   

  1. 1.West China Pharmaceutical School of Sichuan University, Chengdu 610041, China;
    2.Chengdu Medical College, Chengdu 610083, China
  • Received:2007-01-15 Revised:2007-04-29 Online:2007-07-30 Published:1985-06-25

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Abstract:

A method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for analysis and identification of chlorogenic acid along with its related impurities. A Gemini C18 HPLC column (4.6 mm×150 mm，5 μm) was used with acetonitrile-water ( containing 0.1% formic acid) (8∶92, v/v) as mobile phase. Eight related impurities of chlorogenic acid were identified and their structures were determined by using on-line HPLC-MS/MS and photodiode array detector (DAD). The method is quick, simple and can be used directly to identify the structure of unknown trace substances in the sample of chlorogenic acid.

Key words: chlorogenic acid, identification
, related impurities, high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS)