Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2019, Vol. 37 ›› Issue (3): 293-298.DOI: 10.3724/SP.J.1123.2018.11026

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Preparation of hydrophilic, magnetic molecularly imprinted resins of chlorogenic acid and evaluation of its solid-phase extraction performance

PENG Sheng1,2, LI Huan3, SHI Shuyun1,2,3   

  1. 1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China;
    2. National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommiaulmoides, Jishou University, Jishou 416000, China;
    3. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
  • Received:2018-11-18 Online:2019-03-08 Published:2015-01-21
  • Supported by:

    National Natural Science Foundation of China (No. 81673585); Project of Key Laboratory of Hunan Forest Products and Chemical Industry Engineering (No. JDZ201802); Project of National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides (No. NLE201601).

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Abstract:

Hydrophilic, magnetic molecularly imprinted resins (MMIRs) were fabricated inside Fe3O4@mSiO2 pores by the one-pot copolycondensation of chlorogenic acid (CGA), resorcinol, melamine, and formaldehyde. The final porous MMIRs with larger numbers of surface recognition sites were obtained by removing mSiO2. The successful preparation of MMIRs was confirmed using transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometry, thermo-gravimetric analysis, and water contact angle analysis. The analyses suggest that the MMIRs have excellent hydrophilicity and magnetic solid-phase extraction efficiency. The MMIRs had a good adsorption capacity (50.87 mg/g), fast rate of adsorption (equilibrium was attained at 70 min), and specific recognition for CGA. The MMIRs, in combination with high performance liquid chromatography, were directly used for the selective extraction and determination of CGA in Duzhong brick tea. The limit of detection and recovery were 0.7 mg/L and 93.1%-109.4%, respectively, with relative standard deviations of less than 9.6%. The proposed strategy was extremely promising for the facile, rapid, and selective determination of CGA in real aqueous samples.

Key words: chlorogenic acid, complex sample, high performance liquid chromatography (HPLC), hydrophilic molecularly imprinted resin, solid phase extraction (SPE)

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