Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2016, Vol. 34 ›› Issue (4): 401-406.DOI: 10.3724/SP.J.1123.2015.11043

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Liquid chromatography-tandem mass spectrometry analysis for azaspiracids and their metabolites

WU Haiyan1,2, GUO Mengmeng1,2, ZHAO Chunxia1,2, TAN Zhijun1,2, GU Haifeng3, ZHAI Yuxiu1,2, LU Lina1,2   

  1. 1. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
    2. Laboratory of Quality & Safety Risk Assessment for Aquatic Products(Qingdao), Ministry of Agriculture, Qingdao 266071, China;
    3. Third Institute of Oceanography, State Oceanic Administration, Xiamen 361000, China
  • Received:2015-11-26 Online:2016-04-08 Published:2012-11-16
  • Supported by:

    National Natural Science Foundation of China (No. 41106109);Special Funds for the Basic R & D Program in the Central Non-Profit Research Institutes (Nos. 20603022011004, 20603022015017-2).

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Abstract:

A liquid chromatography-tandem mass spectrometry method was developed for the analysis of azaspiracids and their metabolites. Both target metabolites and non-objective compounds detection methods were investigated, and the secondary mass spectra were compared with the standard spectra libraries to achieve high precision qualitative analysis. As a result, 11 metabolites including AZA-2, 3, 6, 11, 12, 16, 17, 28 and 36, as well as the glutathione conjugated metabolites of AZAs were analyzed in toxin-producing algae, accumulated samples of metabolic experiments and positive samples. Conventional metabolites were unbiased detected by target metabolites detection method, and showed a better response to low concentration metabolites. The method has excellent reproducibility, and is easy for operators. It can be used for the routine monitoring of AZAs.

Key words: azaspiracids (AZAs), liquid chromatography-tandem mass spectrometry (LC-MS/MS), metabolites, qualitative analysis

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