Abstract: A rapid and sensitive method was developed for the identification and quantification of stanozolol in hair. After alkaline hydrolysis, the hair sample (10 mg) was extracted with pentane, and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI) and multiple reaction monitoring (MRM). The limit of quantification was found to be 25 pg/mg. After shaving hair on the back of the cavies, they were received after a single intraperitoneal dosing of stanozolol of 60 mg/kg. The hair segments were shaved once every two days for two weeks. The concentration of stanozolol in segmented hair reached the highest at the 10th day. The features of the method are of small sample size, specific, sensitive and suitable for the determination of stanozolol in hair.

Key words: hair , stanozolol, liquid chromatography-tandem mass spectrometry (LC-MS/MS)