Abstract: The method for the analysis of glucosamine hydrochloride in health foods using high performance anion exchange chromatography was established. The determination of glucosamine hydrochloride was performed on an AminoPac PA10 column (2 mm×250 mm) with a pulse amperometric detector with an Au working electrode and an Ag/AgCl reference electrode. The linear range was 0.05-10.0 mg/L, and the detection limit was 0.012 mg/L. The relative standard deviations for the determination of the standard and a sample were 0.69% and 1.38%, respectively. The satisfactory results were obtained for the analysis of health food samples with the proposed method, and the spiked recovery was 96.6%-105.2%. In comparison with the national standard method, the relative deviations of the determination results were -1.4%-1.0%. The method is sensitive and precise. One analysis can be completed within 5 min. This method was available for the determination of glucosamine hydrochloride in health foods.

Key words: glucosamine hydrochloride, health food , pulse amperometric detector, high performance anion exchange chromatography