Abstract: A “one-pot” process for the preparation of organic-silica hybrid capillary monolithic column by concurrently using tetramethoxysilane (TMOS), vinyltrimethoxysilane (VTMS) and the organic monomer, N-(2-(methacryloyloxy)ethyl)dimethyl-octadecylammonium bromide (MDOAB), is described. The polycondensation of alkoxysilanes and the copolymerization of MDOAB and VTMS were subsequently carried out within the confines of a capillary under the proper reaction conditions. The performance of the C18-silica hybrid monolithic column was investigated by capillary electrochromatography and capillary liquid chromatography. In addition, the C18-silica hybrid capillary monolithic column was also applied in the analysis of tryptic digests of bovine serum albumin by capillary liquid chromatography/tandem mass spectrometry (CLC-MS/MS) for demonstrating its potential in proteome analysis. This in situ process of incorporating functional groups into silica monolith provides a new way for the preparation of the organic-silica hybrid monolithic column.

Key words: capillary electrochromatography （CEC）, capillary liquid chromatography （CLC）, one-pot, proteome analysis, monolith, organic-inorganic hybrid