Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride

doi:10.3724/SP.J.1123.2012.11021

Abstract

Abstract:

The online enrichment ability of the restricted-access media (RAM) column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride in plasma was studied. The RAM-HPLC system consisted of an RAM column as enrichment column and a C18 column as analytical column coupled via the column switching technique. The effects of the injection volume on the peak area and the systematic pressure were studied. When the injection volume was less than 100 μL, the peak area increased with the increase of the injection volume. However, when the injection volume was more than 80 μL, the pressure of whole system increased obviously. In order to protect the whole system, 80 μL was chosen as the maximum injection volume. The peak areas of ordinary injection and the large volume injection showed a good linear relationship. The enrichment ability of RAM-HPLC system was satisfactory. The system was successfully used for the separation and detection of the trace benazepril hydrochloride in rat plasma after its administration. The sensitivity of HPLC can be improved by RAM pre-enrichment. It is a simple and economic measurement method.

Key words: benazepril hydrochloride, direct injection, high-performance liquid chromatography (HPLC), online enrichment, restricted-access media (RAM), serum

CLC Number:

O658

ZHANG Xiaohui, WANG Rong, XIE Hua, YIN Qiang, LI Xiaoyun, JIA Zhengping, WU Xiaoyu, ZHANG Juanhong, LI Wenbin. Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride[J]. Chinese Journal of Chromatography, 2013, 31(5): 451-455.

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