Rapid simultaneous screening and detection of ten anticoagulant rodenticides in foods by ultra-high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2012.11026

Abstract

Abstract:

A rapid method for the simultaneous screening and detection of ten anticoagulant rodenticides in foods was developed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After the extraction by acetonitrile and clean-up by QuEChERS, the extract was separated on a Poroshell 120 EC-C18 column (100 mm×2.1 mm, 2.7 μm) with the gradient elution of 5 mmol/L ammonium acetate and acetonitrile. The detection was carried out by UPLC-MS/MS using a negative electrospray ionization interface in multiple reaction monitoring (MRM) mode. The ten anticoagulant rodenticides showed a good linear relationship (r>0.99) in the range of 5-500 μg/L. In the four samples of chili sauce, flour, vinegar and soy sauce, the spiked recoveries were in the range of 72.6%-112%, and the relative standard deviations (RSD) were all not more than 11.2%. The limits of detection (LOD) were all in the range of 0.5-4.5 μg/kg. This method is rapid, simple, sensitive, accurate and of good specificity for the satisfactory rapid screening and detection of the ten anticoagulant rodenticides in the sudden public health security events.

Key words: anticoagulant rodenticides, foods, ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

CLC Number:

O658

ZHU Feng, LIU Hualiang, CHEN Bei, RONG Weiguang, MA Yongjian. Rapid simultaneous screening and detection of ten anticoagulant rodenticides in foods by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2013, 31(5): 473-476.

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