Determination of urinary cotinine of children exposed to passive smoking by stable isotope dilution gas chromatography-triple quadrupole mass spectrometry

doi:10.3724/SP.J.1123.2014.01023

Abstract

Abstract:

An analytical method for the determination of urinary cotinine of children exposed to passive smoking was established based on stable isotope dilution by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The samples were extracted and purified with chloroform. The extracts were determined by GC-MS/MS in multiple reaction monitoring (MRM) mode. The cotinine-d₃ as an isotope internal standard was applied to quantify and confirm the urinary cotinine of children exposed to passive smoking. The method had a good linearity from 0.1 μg/L to 10 μg/L with the correlation coefficient (r)>0.998. The recoveries of the cotinine in blank urine were from 79.2% to 112.8% at spiked levels of 0.1, 1.0 and 10 μg/L, with relative standard deviations (RSDs) from 2.1% to 5.8%. The limit of quantification (LOQ) of the method was 0.1 μg/L. The developed method is accurate, sensitive, rapid and can be applied to detect urinary cotinine of children exposed to passive smoking at home.

Key words: children, cotinine, gas chromatography-tandem mass spectrometry (GC-MS/MS), isotope dilution, passive smoking, urine

CLC Number:

O658

WANG Yun, HUANG Zhiqiang, YE Ying, ZHANG Ying, XIAO Shuiyuan. Determination of urinary cotinine of children exposed to passive smoking by stable isotope dilution gas chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(6): 658-661.

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