Microdevice for the investigation of high-glucose induced lifespan and the protective effect of polydatin in C. elegans

doi:10.3724/SP.J.1123.2015.10009

Abstract

Abstract:

Caenorhabditis elegans (C. elegans) has been widely used as a model organism for biomedical research due to its sufficient homology with human at molecular or genomic level. In this work, we describe a microfluidic device not only to investigate the response of C. elegans including lifespan and oxidative stress, but also to evaluate the protective effect of polydatin induced by high-glucose condition. It was found that the mean lifespan of worms was significantly reduced and the oxidative stress protein GST-4 was increased in worms that are subjected to high glucose. However, a certain dose of polydatin could weaken the increased oxidative stress induced by high-glucose and extend the lifespan, indicating the protective effect of polydatin against the toxic of high-glucose. The established approach is simple to operate, easy for realtime imaging and multiparatemer evaluations in parallel, providing a potential platform for drug evaluation/screening in a high throughput format at single animal resolution.

Key words: Caenorhabditis elegans, glucose, microfluidic, polydatin

CLC Number:

O658

ZHU Guoli, YIN Fangchao, WANG Li, ZHANG Min, JIANG Lei, QIN Jianhua. Microdevice for the investigation of high-glucose induced lifespan and the protective effect of polydatin in C. elegans[J]. Chinese Journal of Chromatography, 2016, 34(2): 140-145.

References

[1] Brenner S. Genetics, 1974, 77(1): 71
[2] Rille D L, Blumenthal T, Meyer B J, et al. C. elegansⅡ. New York: Cold Spring Harbor Laboratory Press, 1997
[3] The C. elegans Sequencing Consortium. Science, 1998, 282: 2012
[4] Beale E, Li G, Tan M W, et al. Appl Environ Microbiol, 2006, 72(7): 5135
[5] Schlotterer A, Kukudov G, Bozorgmehr F, et al. Diabetes, 2009, 58(11): 2450
[6] Lee S J, Murphy C T, Kenyon C. Cell Metab, 2009, 10(5): 379
[7] Tauffenberger A, Parker J A. PLoS Genet, 2014, 10(5): e1004346
[8] Schulz T J, Zarse K, Voigt A, et al. Cell Metab, 2007, 6(4): 280
[9] Schmeisser S, Zarse K, Ristow M. Horm Metab Res, 2011, 43(10): 687
[10] Cichewicz R H, Kouzi S A. J Nat Prod, 1998, 61: 1313
[11] Fabris S, Momo F, Ravagnan G, et al. Biophys Chem, 2008, 135: 76
[12] Perez A I R, Gomez M I, Lamuela-Raventos R M, et al. J Agric Food Chem, 1999, 47: 1533
[13] Miao Q, Wang S, Miao S, et al. Phytomed, 2011, 19: 8
[14] Ji H, Zhang X, Du Y, et al. Brain Res Bull, 2012, 87: 50
[15] Lanzilli G, Cottarelli A, Nicotera G, et al. Inflammation, 2011, 35: 240
[16] Shu S, Ling Z Y, Mao Y, et al. BMC Cell Biol, 2011, 12: 31
[17] Huang Z S, Wang Z W, Liu M P, et al. World J Gastroenterol, 1999, 5: 41
[18] Wen H, Shi W, Qin J. BioMed Microdevice, 2012, 14(4): 721
[19] Wen H, Gao X, Qin J. Integ Bio, 2014, 6(1): 35
[20] Cabreiro F, Au C, Leung K Y, et al. Cell, 2013, 153(1): 228
[21] Ma H, Jiang L, Shi W W, et al. Biomicrofluidics, 2009, 3(4): 44114
[22] Shi W W, Wen H, Lu Y, et al. Lab Chip, 2010, 10(21): 2855
[23] Kim N, Dempsey C M, Zoval J V, et al. Sens Actuators B, 2007, 122(2): 511
[24] Chung K H, Crane M M, Lu H. Nat Methods, 2008, 5(7): 637
[25] Crane M M, Stirman J N, Ou C Y, et al. Nat Methods, 2012, 9(10): 977
[26] Siddique R, Thakor N. J R Soc Interface, 2014, 11(90): 20130676
[27] Hulme S E, Shevkoplyas S S, McGuigan A P, et al. Lab Chip, 2010, 10(5): 589
[28] Shi W W, Qin J H, Ye NN, et al. Lab Chip, 2008, 8(9): 1432
[29] Wen H, Yu Y, Zhu G L, et al. Lab Chip, 2015, 15(8): 1905
[30] Lin B C, Qin J H. 2nd. An Illustration to Lab-on-a-Chip Laboratory on Microfluidic Chip. Beijing: Science Press, 2008 林炳承, 秦建华. 图解微流控芯片实验室, 2版. 北京: 科学出版社, 2008
[31] Emmons S W, Klass M R, Hirsh D. Proc Natl Acad Sci USA, 1979, 76(3): 1333