Research advances of aptamer selection for whole cell

doi:10.3724/SP.J.1123.2015.12015

Abstract

Abstract:

Aptamers are single stranded DNA (ssDNA) or RNA that may bind to small molecules, proteins, cells, microorganisms, and other targets. Typically, aptamers are generated by a selection process referred to as systematic evolution of ligands by exponential enrichment (SELEX). Aptamers that bind with high affinity and specificity to proteins that reside on the cell surface have potential utility in the fields of biosenser, molecular imaging, medical diagnosis, drug delivery, and disease treatment. However, till now, available aptamers are very limited mainly due to the complex and difficulty screening of aptamer and their poor properties. Using purified cell surface proteins as target has the disadvantage of changing structure. When target proteins are present in a modified state, the isolated aptamers might not recognize the natural structure of some proteins. Aptamers screening targeting whole cell has the potential to be used as cell probe for directing whole cell analysis. It does not need to understand the molecular structure of the cell surface protein and maintain the natural state of cells in the screening process. This review mainly focuses on the advances of aptamer selection for whole cell from 2008 to 2015. The contents include the classification of target cells, design of library, methods for cell-ssDNA complex separation and affinity characterization. The sequences of aptamers reported are listed.

Key words: aptamers, cells, review, selection, systematic evolution of ligands by exponential enrichment (SELEX)

CLC Number:

O658

LIU Pinduo, QU Feng. Research advances of aptamer selection for whole cell[J]. Chinese Journal of Chromatography, 2016, 34(4): 382-388.

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