Isolation and purification of galangin and kaempferide from Alpinia officinarum Hance by preparative high-performance liquid chromatography

doi:10.3724/SP.J.1123.2016.03009

Abstract

Abstract:

A method of the preparation of galangin and kaempferide from Alpinia officinarum Hance flavonoids using preparative high-performance liquid chromatography (Prep-HPLC) was established. After the Alpinia officinarum Hance flavonoids was purified by HPD-600 resin-based column chromatography, the flavonoid components were separated by Prep-HPLC on a Venusil XBP-C18 column (250 mm×21 mm, 5.0 μ m). The mixture of methanol and 0.6% (v/v) acetic acid solution (58:42, v/v) was used as mobile phase at a flow rate of 7.0 mL/min. The sample volume was 700 μ L and the UV detection wavelength was 360 nm. With MS and NMR, the structures of component I and component II of the Alpinia officinarum Hance flavonoids were identified. The average relative molecular mass of the component I was 269, the same as galangin; and that of the component II was 299, the same as kaempferide. HPLC analysis showed that the purities of galangin and kaempferide were 99.5% and 99.7% respectively. The method is simple and of low toxicity. It can be applied to the separation and preparation of galangin and kaempferide.

Key words: Alpinia officinarum Hance, galangin, isolation, kaempferide, preparative high-performance liquid chromatography (Prep-HPLC), purification

CLC Number:

O658

HOU Hongrui, HUANG Jidong, CHEN Ling, HUANG Yanran, WANG Chunxiao. Isolation and purification of galangin and kaempferide from Alpinia officinarum Hance by preparative high-performance liquid chromatography[J]. Chinese Journal of Chromatography, 2016, 34(6): 591-595.

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