Determination of L-carnitine in dairy products by high performance liquid chromatography coupled with pre-column derivatization

doi:10.3724/SP.J.1123.2016.03027

Abstract

Abstract:

A method has been established for the determination of L-carnitine in dairy products by high performance liquid chromatography (HPLC) coupled with pre-column derivatization. The samples were extracted by ultrasonator with 0.1 mol/L HCl as extraction solvent, and purified by a cation exchange solid phase extraction column, undergone a substitution reaction with L-propionamide-β-naphthylamine catalyzed by triethylamine and butyl chlorocarbonate. Then, the compound was detected by HPLC, with a diode array detection (DAD) as quantitative method. A linear calibration curve was obtained in the mass concentration range of 0.250-50.0 mg/L. The linear equation was y=164.4x-11.3 with the correlation coefficient of 0.9998, and the recoveries were 84.3%~86.0% with the relative standard deviations (RSDs) of 1.93%~3.18%. The limit of detection (LOD) was 10 mg/kg, and the limit of quantification (LOQ) was 25 mg/kg. The proposed procedure was then applied to the analysis of 20 real samples collected from China, and the contents of L-carnitine were 53-163 mg/kg. This method is rapid, simple, accurate and suitable for the determination of L-carnitine in dairy products.

Key words: dairy products, derivatization, high performance liquid chromatography (HPLC), L-carnitine

CLC Number:

O658

WANG Qiaoling, YU Yue, XU Dunming, ZHANG Zhigang, FANG Enhua, FENG Feng, ZHANG Feng, CHU Xiaogang. Determination of L-carnitine in dairy products by high performance liquid chromatography coupled with pre-column derivatization[J]. Chinese Journal of Chromatography, 2016, 34(7): 697-702.

References

[1] Yang J. Agricultural Engineering, 2012, 2(1):54 杨静. 农业工程, 2012, 2(1):54
[2] Zhou S F, He Z Q, Liu J P, et al. Acta Nutrimenta Sinica, 1997, 19(2):146 周书凤, 何志谦, 刘建平, 等. 营养学报, 1997, 19(2):146
[3] Chen J, Zhong X L, Yu Y H. Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2013, 49(1):6 陈军, 钟新林, 余彦海. 理化检验:化学分册, 2013, 49(1):6
[4] GB 14880-2012
[5] GB 10765-2010
[6] GB 10767-2010
[7] Zuo H X, Zhang Y B, Huang M M, et al. Science and Technology of Food Industry, 2012, 35(2):53 左惠心, 张玉斌, 黄明明, 等. 食品工业科技, 2012, 35(2):53
[8] Wang T X, Xiong W M, Lin M Y. Fujian Analysis & Testing, 2012, 21(1):52 王天西, 熊文明, 林梦勇. 福建分析测试, 2012, 21(1):52
[9] Xu P. Life Science Instruments, 2013(5):45 徐萍. 生命科学仪器, 2013(5):45
[10] Jin S M, Zhao Z H, Zhu H, et al. Cereal and Food Industry, 2011, 18(5):54 金世梅, 赵志红, 朱慧, 等. 粮食与食品工业, 2011, 18(5):54
[11] Zou X L, Zhou Y Y, Qiao R, et al. Journal of Hygiene Research, 2011, 40(3):358 邹晓莉, 周艳阳, 乔蓉, 等. 卫生研究, 2011, 40(3):358
[12] Gan B B, Li S H. Chinese Journal of Health Laboratory Technology, 2010(7):1688 甘宾宾, 黎少豪. 中国卫生检验杂志, 2010(7):1688
[13] Xu L M, Wang Z Z, Bi Y A, et al. Strait Pharmaceutical Journal, 2009, 21(11):46 徐连明, 王振中, 毕宇安, 等. 海峡药学, 2009, 21(11):46
[14] Zhu Q, Su L K, Zheng J G, et al. Journal of Instrumental Analysis, 2012, 31(3):355 竺琴, 苏流坤, 郑家概, 等. 分析测试学报, 2012, 31(3):355
[15] Xu D M, Chen Y, Zhang J, et al. Journal of Instrumental Analysis, 2016, 35(1):1 徐敦明, 陈燕, 张缙, 等. 分析测试学报, 2016, 35(1):1
[16] Liu Y M, Xue X, Liu G Q, et al. Chinese Journal of Chromatography, 2015, 33(9):943 刘艳明, 薛霞, 刘国强, 等. 色谱, 2015, 33(9):943
[17] Miyuki K, Yoshio M, Hiroyuki N. J Chromatogr A, 1999, 857:127
[18] Sascha F L, Hans A. J Pharm Biomed Anal, 2002, 30:209