Rapid detection of 248 pesticide residues in vegetables by ultra high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2016.04004

Abstract

Abstract:

A multiresidue analytical method for rapid detection of 248 pesticide residues in vegetables using an ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) technique was successfully developed. The vegetable samples were extracted by acetonitrile. After salting out, the solvent extracts were analyzed by UHPLC-MS/MS directly. And further purification was not needed. The positive and negative ion modes and multiple reaction monitoring (MRM) mode were used to identify and quantify the 248 pesticide residues in vegetables. The results showed that 245 pesticides had good linearity within their respective linear ranges (r>0.99). Except four pesticides (carbosulfan, cyromazine, tribenuron-methyl, quinclorac), the average recoveries of the other 244 pesticides at three spiked levels ranged from 63.0% to 126.4% with the relative standard deviations (RSDs) from 0.5% to 26.7%. The limits of quantification of this method were in the range of 0.001-0.030 mg/kg. In comparison with other pretreatment methods for pesticide residues, this method not only shortened the time of sample pretreatment, but also enhanced significantly the recoveries of pesticides. The results in this study suggested that it is a simple, rapid, sensitive and accurate method for pesticide residue determination, and suitable for rapid screening of pesticide residues in vegetables.

Key words: pesticide residues, ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), vegetables

CLC Number:

O658

LI Lingyun, XU Xiaomin, LIN Huan, ZHAO Wen, YE Rong, HUANG Xiaodong, ZHENG Shuning, LIU Xinyan, LIU Su, XU Donghui. Rapid detection of 248 pesticide residues in vegetables by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(9): 835-849.

References

[1] Zheng Y Q. Plant Protection, 2013, 39(5): 90 郑永权. 植物保护, 2013, 39(5): 90
[2] Chen C. [PhD Dissertation]. Beijing: Chinese Academy of Agricultural Sciences, 2014 陈晨. [博士学位论文]. 北京: 中国农业科学院, 2014
[3] Zhong W K, Hao J, Fan Y B, et al. Chinese Journal of Analytical Chemistry, 2000, 28(7): 904 仲维科, 郝戬, 樊耀波, 等. 分析化学, 2000, 28(7): 904
[4] Cao J, Pang G F, Wang M L, et al. Chinese Journal of Chromatography, 2010, 28(6): 579 曹静, 庞国芳, 王明林, 等. 色谱, 2010, 28(6): 579
[5] Gong L P, Shi F, Jiang S Y, et al. Chinese Journal of Chromatography, 2015, 33(4): 408 巩丽萍, 石峰, 姜树银, 等. 色谱, 2015, 33(4): 408
[6] Li X S. Food Science, 2003, 24(6): 124 李新社. 食品科学, 2003, 24(6): 124
[7] Wu Y, Jiang B, Xu Y G, et al. Chinese Journal of Chromatography, 2015, 33(3): 228 吴岩, 姜冰, 徐义刚, 等. 色谱, 2015, 33(3): 228
[8] Wang L Z, Zhou Y, Chen Y, et al. Chinese Journal of Chromatography, 2012, 30(2): 146 王连珠, 周昱, 陈泳, 等. 色谱, 2012, 30(2): 146
[9] Lin T, Shao J L, Liu X Y, et al. Chinese Journal of Chromatography, 2015, 33(3): 235 林涛, 邵金良, 刘兴勇, 等. 色谱, 2015, 33(3): 235
[10] Da J, Wang G L, Cao J, et al. Chinese Journal of Chromatography, 2015, 33(8): 830 达晶, 王钢力, 曹进, 等. 色谱, 2015, 33(8): 830
[11] Liu Y B, Fan M T, Liang J, et al. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(4): 61 刘玉波, 樊明涛, 梁俊, 等. 西北农业学报, 2009, 18(4): 61
[12] Cao J, Huang X F, Wang T Q, et al. Chinese Journal of Analysis Laboratory, 2009, 28(Suppl): 42 曹军, 黄月芳, 王太全, 等. 分析试验室, 2009, 28(Suppl): 42
[13] Dong J, Gong X M, Zhang L, et al. Chinese Journal of Analysis Laboratory, 2008, 27(3): 91 董静, 宫小明, 张立, 等. 分析试验室, 2008, 27(3): 91
[14] Ma L L, Jia L, Zhou X R, et al. Chinese Journal of Chromatography, 2014, 32(6): 635 马立利, 贾丽, 周欣然, 等. 色谱, 2014, 32(6): 635
[15] Jia W, Huang J R, Ling Y, et al. Journal of Instrumental Analysis, 2013, 32(1): 9 贾玮, 黄峻榕, 凌云, 等. 分析测试学报, 2013, 32(1): 9
[16] Chen X, Cheng L, Qu S C, et al. Chinese Journal of Chromatography, 2015, 33(10): 1080 陈溪, 程磊, 曲世超, 等. 色谱, 2015, 33(10): 1080
[17] Xu J, Wang L, Huang H J, et al. Chinese Journal of Chromatography, 2015, 33(3): 242 徐娟, 王岚, 黄华军, 等. 色谱, 2015, 33(3): 242
[18] Zheng S N, Li L Y, Lin H, et al. Chinese Journal of Chromatography, 2013, 31(1): 71 郑姝宁, 李凌云, 林桓, 等. 色谱, 2013, 31(1): 71
[19] Ma Z L, Zhao W, Li L Y, et al. Chinese Journal of Chromatography, 2013, 31(3): 228 马智玲, 赵文, 李凌云, 等. 色谱, 2013, 31(3): 228
[20] Lü B, Chen D W, Miao H, et al. Journal of Instrumental Analysis, 2015, 34(6): 639 吕冰, 陈达炜, 苗虹, 等. 分析测试学报, 2015, 34(6): 639
[21] Cheng Z, Zhang R, Liu W H, et al. Chinese Journal of Chromatography, 2014, 32(1): 57 程志, 张蓉, 刘韦华, 等. 色谱, 2014, 32(1): 57
[22] Su J F, Zhong M S, Chen J, et al. Journal of Instrumental Analysis, 2015, 34(6): 625 苏建峰, 钟茂生, 陈晶, 等. 分析测试学报, 2015, 34(6): 625
[23] Sun J, Chen G H, Wang K. Chinese Journal of Analytical Chemistry, 2010, 38(8): 1151 孙娟, 陈冠华, 王坤, 等. 分析化学, 2010, 38(8): 1151
[24] Cao X Y, Pang G F, Jin L H, et al. Chinese Journal of Chromatography, 2015, 33(4): 389 曹新悦, 庞国芳, 金铃和, 等. 色谱, 2015, 33(4): 389
[25] Zhao Z Y, Shi Z H, Kang J, et al. Chinese Journal of Chromatography, 2013, 31(4): 372 赵志远, 石志红, 康健, 等. 色谱, 2013, 31(4): 372
[26] Zhang F, Huang Z Q, Zhang Y, et al. Chinese Journal of Chromatography, 2010, 28(4): 348 张帆, 黄志强, 张莹, 等. 色谱, 2010, 28(4): 348
[27] Wong J, Hao C Y, Zhang K, et al. J Agric Food Chem, 2010, 58: 5897
[28] Sack C, Smoker M, Chamkasem N, et al. J Agric Food Chem, 2011, 59: 6383
[29] Zhang K, Wong J W, Yang P, et al. J Agric Food Chem, 2011, 59: 7636
[30] Kmellár B, Fodor P, Pareja L, et al. J Chromatogr A, 2008, 1215: 37
[31] Wang J, Chow W, Leung D. Anal Bioanal Chem, 2010, 396: 1513
[32] Chambers E, Wagrowski-Diehl D M, Lu Z L, et al. J Chromatogr B, 2007, 852(1/2): 22