Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2016, Vol. 34 ›› Issue (10): 992-997.DOI: 10.3724/SP.J.1123.2016.06048

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Optimization of the extraction method for high performance liquid chromatography analysis of trace di(2-ethylhexyl)phthalate in drinking water by response surface methodology and the migration regularity

WU Fei1,2, LÜ Xiaodan1,2, TANG Junjie1,2, CHEN Qixian1, PENG Xuetang1, CHEN Qidan1,2   

  1. 1. Zhuhai College of Jilin University, Zhuhai 519041, China;
    2. Zhuhai Institute of Supramolecular Materials, Jilin University, Zhuhai 519041, China
  • Received:2016-06-29 Online:2016-10-08 Published:2013-04-03
  • Supported by:

    National Natural Science Foundation of China (No. 21307039); Foundation for Distinguished Young Talents in Higher Education of Guangdong China (No. 2013LYM_0120); Foundation for Distinguished Young Teachers in Higher Education of Guangdong China (No. YQ2014231); Natural Science Foundation of Guangdong China (No. 2016A03031383).

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Abstract:

A simple and rapid method for the determination of trace di(2-ethylhexyl)phthalate (DEHP) in drinking water was developed and the migration regularity was studied. In this work, the migration of DEHP from plastic bottle products of supermarket was studied by high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV), and the extraction conditions were optimized by response surface methodology (RSM). With the effect of NaCl, DEHP in drinking water sample was extracted in n-hexane by vortex, dried and concentrated by N2, and then dissolved by methanol. The filtrate was injected into an HPLC-UV system for the analysis. The optimized extraction conditions were NaCl of 16 g/L, the vortex time of 90 s and the volume of n-hexane of 5.28 mL. Under the optimized conditions, the experimental value and the predicted value of the recoveries of DEHP were 92.97% and 92.91%, respectively, and the difference was only 0.063%. The limit of detection was 0.006 mg/L, the linear range was 0.01-15 mg/L (R=0.9996), and the relative standard deviation (RSD) was 0.015%-2.45%. In addition, the real water sample in plastic bottles from local supermarket was determined by the method and the migration regularity of trace DEHP in drinking water from plastic container under different temperature and time conditions was studied. The results showed that the application of RSM in the selection of optimal conditions for trace analysis is feasible, low-cost and eco-friendly.

Key words: di(2-ethylhexyl)phthalate (DEHP), drinking water, high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV), migration regularity, response surface methodology (RSM)

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