Determination of nine N-nitrosamines in animal derived foods by dispersive solid-phase extraction-gas chromatography-tandem mass spectrometry

Abstract

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doi:10.3724/SP.J.1123.2016.07012

Abstract:

A method has been developed and validated for the determination of nine N-nitrosamines in animal derived food using dispersive solid-phase extraction (dSPE) coupled with isotope dilution-gas chromatography-tandem mass spectrometry (GC-MS/MS). The samples were extracted by acetonitrile. The extracts were purified using mixed adsorbent by dSPE and then concentrated. The analytes were separated on a DB-WAX polar capillary column, and ionized by EI source. The data were collected by multiple reaction monitoring (MRM) mode, followed by qualitative screening and quantitative analysis. The correlation coefficients of linear calibration curves were over 0.99 in the corresponding linear ranges. Four kinds of typical animal derived foods were spiked at three levels. The average recoveries of the nine N-nitrosamines ranged from 62.5% to 118% with RSDs of 2.11%-25.6%. The limits of detection (LODs, S/N=3) were 0.02-0.31 μg/kg. The method is of low cost, sensitive, reliable, and suitable for the determination of nine N-nitrosamines in animal derived foods.

Key words: N-nitrosamines, animal derived foods, dispersive solid-phase extraction (dSPE), gas chromatography-tandem mass spectrometry (GC-MS/MS), isotope dilution

CLC Number:

O658

ZHAO Bo, DENG Meilin, YANG Xiaoshan, DUAN Yunpeng, WU Xiaoqin, TU Dawei. Determination of nine N-nitrosamines in animal derived foods by dispersive solid-phase extraction-gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(10): 998-1004.

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错误：java.util.ConcurrentModificationException
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exception:java.util.ConcurrentModificationException
cause:null
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stackTrace:java.util.ConcurrentModificationException at java.util.HashMap$HashIterator.nextNode(HashMap.java:1442) at java.util.HashMap$KeyIterator.next(HashMap.java:1466) at com.lyt.util.filter.CountDayBrowseFilter.docurrent(CountDayBrowseFilter.java:99) at com.lyt.util.filter.CountDayBrowseFilter.doFilter(CountDayBrowseFilter.java:43) at com.lyt.util.HttpFilter.doFilter(HttpFilter.java:46) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at org.tuckey.web.filters.urlrewrite.RuleChain.handleRewrite(RuleChain.java:164) at org.tuckey.web.filters.urlrewrite.RuleChain.doRules(RuleChain.java:141) at org.tuckey.web.filters.urlrewrite.UrlRewriter.processRequest(UrlRewriter.java:90) at org.tuckey.web.filters.urlrewrite.UrlRewriteFilter.doFilter(UrlRewriteFilter.java:406) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.lyt.util.filter.UrlRedirectFilter.doFilter(UrlRedirectFilter.java:52) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.lyt.util.filter.CsrfFilter.doFilter(CsrfFilter.java:198) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.magtech.filter.UserGroupMapFilter.doFilter(UserGroupMapFilter.java:39) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.magtech.filter.XssFilter.doFilter(XssFilter.java:66) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.lyt.util.web.CharacterEncodingFilter.doFilter(CharacterEncodingFilter.java:64) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.lyt.util.filter.FrameTaoFilter.doFilter(FrameTaoFilter.java:29) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.lyt.util.filter.JournalIDFilter.doFilter(JournalIDFilter.java:80) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.lyt.util.filter.ReadRequestRefererFilter.doFilter(ReadRequestRefererFilter.java:32) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at com.lyt.util.filter.AuthenticationFilter.doFilter(AuthenticationFilter.java:107) at com.lyt.util.HttpFilter.doFilter(HttpFilter.java:46) at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:193) at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:166) at org.apache.catalina.core.StandardWrapperValve.invoke(StandardWrapperValve.java:196) at org.apache.catalina.core.StandardContextValve.invoke(StandardContextValve.java:97) at org.apache.catalina.authenticator.AuthenticatorBase.invoke(AuthenticatorBase.java:542) at org.apache.catalina.core.StandardHostValve.invoke(StandardHostValve.java:135) at org.apache.catalina.valves.ErrorReportValve.invoke(ErrorReportValve.java:81) at org.apache.catalina.valves.RemoteIpValve.invoke(RemoteIpValve.java:769) at org.apache.catalina.valves.AbstractAccessLogValve.invoke(AbstractAccessLogValve.java:698) at org.apache.catalina.core.StandardEngineValve.invoke(StandardEngineValve.java:78) at org.apache.catalina.connector.CoyoteAdapter.service(CoyoteAdapter.java:364) at org.apache.coyote.http11.Http11Processor.service(Http11Processor.java:624) at org.apache.coyote.AbstractProcessorLight.process(AbstractProcessorLight.java:65) at org.apache.coyote.AbstractProtocol$ConnectionHandler.process(AbstractProtocol.java:831) at org.apache.tomcat.util.net.NioEndpoint$SocketProcessor.doRun(NioEndpoint.java:1650) at org.apache.tomcat.util.net.SocketProcessorBase.run(SocketProcessorBase.java:49) at org.apache.tomcat.util.threads.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1191) at org.apache.tomcat.util.threads.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:659) at org.apache.tomcat.util.threads.TaskThread$WrappingRunnable.run(TaskThread.java:61) at java.lang.Thread.run(Thread.java:748)

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