Simultaneous determination of 73 flavor components in cut tobacco by gas chromatography-triple quadrupole mass spectrometry

doi:10.3724/SP.J.1123.2016.10047

Abstract

Abstract:

A method for simultaneous determination of 73 flavor components in cut tobacco by gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed. The samples were extracted with anhydrous ether, and the ether extracts were separated on a DB-5MS column (30 m×0.25 mm, 0.25 μm). The interference of complex matrix and peak overlapping of various flavor components were reduced by means of optimizing tandem mass spectrometry parameters. The extracts were analyzed by GC-MS/MS under multiple reaction monitoring (MRM) mode, and internal standard method was applied to quantify the 73 flavor components. The results showed that all the flavor components had good linearity in certain ranges with the correlation coefficients (r²) greater than 0.99. The recoveries of the 73 flavor components at low, middle and high levels ranged from 70.0% to 122.3% with the relative standard deviations (RSDs) of 1.6%-20.4%. The developed method is easy, accurate and sensitive, and it can be applied to the determination of the 73 flavor components in cut tobacco.

Key words: cut tobacco, flavor components, gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS), multipe reaction monitoring (MRM)

CLC Number:

O658

XU Yong, JIANG Ciqing, LI Chao, WU Yiqin, ZHANG Dan, ZOU Nan, ZHANG Chengming, QIN Yunhua, MIAO Mingming, JIANG Wei. Simultaneous determination of 73 flavor components in cut tobacco by gas chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(4): 445-452.

References

[1] Wang Y, Sun W L, Su Q D, et al. Tobacco Science and Technology, 2013(7):43王晔, 孙文梁, 苏庆德, 等. 烟草科技, 2013(7):43
[2] Chu G H, Lu M H, Shu M, et al. Flavour Fragrance Cosmetics, 2006(1):17储国海, 陆明华, 舒明, 等. 香料香精化妆品, 2006(1):17
[3] Kim K P, Zlatkis A, Park J W, et al. Chromatographia, 1982, 15(9):559
[4] LaVoie E J, Tucciarone P, Kagan M, et al. J Agric Food Chem, 1989, 37(1):154
[5] Peng F M, Sheng L Q, Liu B Z, et al. J Chromatogr A, 2004, 1040(1):1
[6] Huang L F, Zhong K J, Sun X J, et al. Anal Chem Acta, 2006, 575(2):236
[7] Teixeira S, Mendes A, Alves A, et al. Anal Chem Acta, 2007, 584(2):439
[8] Wu L J, Duan J, Li C Z, et al. Journal of Analytical Science, 2012, 28(6):807吴丽君, 段佳, 李春子, 等. 分析科学学报, 2012, 28(6):807
[9] Zhu X L, Huang L, Li P P, et al. Analytical Instrumentation, 2011(5):13朱晓兰, 黄兰, 李盼盼, 等. 分析仪器, 2011(5):13
[10] Huang L F, Ma N N, Guo F Q, et al. Chinese Journal of Spectroscopy Laboratory, 2008, 25(4):572黄兰芳, 马南南, 郭方遒, 等. 光谱实验室, 2008, 25(4):572
[11] Li F, Cui C Z, Shi Z H, et al. Agriculture and Technology, 2015, 35(5):41李锋, 崔成哲, 史中华, 等. 农业与技术, 2015, 35(5):41
[12] Yang Q, Tuo S X, Huang Y B, et al. Physical Testing and Chemical Analysis (Part B:Chemical Analysis), 2015, 51(5):616杨琼, 庹苏行, 黄勇兵, 等. 理化检验(化学分册), 2015, 51(5):616
[13] Merckel C, Pragst F, Ratzinger A, et al. J Chromatogr A, 2006, 1116(1/2):10
[14] Ye Q. J Chromatogr A, 2008, 1213(2):239
[15] Xu Z G, Zheng L. Journal of Zhejiang University (Science Edition), 2006, 33(2):192徐子刚, 郑琳. 浙江大学学报(理学版), 2006, 33(2):192
[16] Ruiz-Rodriguez A, Bronze M R, da Ponte M N. J Supercrit Fluid, 2008, 45(2):171
[17] Zhang L, Liu S F, Wang X Y, et al. Tobacco Science and Technology, 2013(4):63张丽, 刘绍锋, 王晓瑜, 等. 烟草科技, 2013(4):63
[18] Qin G J, Rao X F, Yang Y H, et al. Guangzhou Chemical Industry, 2015, 43(11):116覃光炯, 饶雄飞, 杨艳华, 等. 广州化工, 2015, 43(11):116
[19] Ji H W, Yang J G, Wang F, et al. Physical Testing and Chemical Analysis (Part B:Chemical Analysis), 2014, 50(9):1188姬厚伟, 杨敬国, 王芳, 等. 理化检验(化学分册), 2014, 50(9):1188
[20] Liu J Y, Zhao Y, Yang H W, et al. Journal of Natural Science of Hunan Normal University, 2011, 34(2):54刘金云, 赵瑜, 杨华武, 等. 湖南师范大学自然科学学报, 2011, 34(2):54
[21] Lou X H, Gao C C, Zhu W J, et al. Tobacco Science and Technology, 2013(8):45楼小华, 高川川, 朱文静, 等. 烟草科技, 2013(8):45
[22] Liu X Q, Tong L, Meng W T, et al. Chinese Journal of Chromatography, 2015, 33(8):869刘小勤, 佟玲, 孟文婷, 等. 色谱, 2015, 33(8):869
[23] Wang J, Ai L F, Ma Y S, et al. Chinese Journal of Chromatography, 2015, 33(11):1175王敬, 艾连峰, 马育松, 等. 色谱, 2015, 33(11):1175