Determination of choline chloride in milk powder and jelly by solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2016.12024

Abstract

Abstract:

An analytical method was developed for the determination of choline chloride in milk powder and jelly by solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were centrifuged after hydrolyzation 3 h in 10 mL 0.02 mol/L ammonium acetate (glacial acetic acid adjust pH to 3.0). The supernatant was cleaned up by a DIKMA ProElut PLS column (60 mg/3 mL). The target compounds were separated by an Agilent ZORBAX 300 SCX column (150 mm×2.1 mm, 5 μm). Qualitative and quantitative analyses were carried out by multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI⁺). The limit of detection (LOD, S/N=3) and the limit of quantitation (LOQ, S/N=10) were 0.15 mg/kg and 0.50 mg/kg, respectively. The recoveries of choline chloride spiked in milk powder and jelly were 70.8%-100.2%, with RSDs no more than 6.83% (n=6). The standard curves showed good linearity in the concentration range from 0.05 to 8.0 mg/L with correlation coefficient (r) of 0.996. The method was applied to detection of commercially available choline chloride in milk powder and jelly. The contents of choline chloride in real milk power and jelly samples were 251.0-2448 mg/kg and 0.261-0.314 mg/kg, respectively. The developed method is accurate, reliable, sensitive, and suitable for the determination of choline chloride in milk powder and jelly.

Key words: choline chloride, jelly, milk powder, solid phase extraction (SPE), ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

CLC Number:

O658

WANG Hui, LIU Jiang, LI Xihui, CAO Yang, HUANG Xiaobei, LI Yongqiang. Determination of choline chloride in milk powder and jelly by solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(5): 558-562.

References

[1] Cao W J, Cui H, Shen B Z, et al. Modern Scientific Instruments, 2012(6):162曹文军, 崔晗, 沈葆真, 等. 现代科学仪器, 2012(6):162
[2] HG/T 2941-2004
[3] Zhong H J, Wei H. Feed Review, 2003(11):20钟红舰, 魏红. 饲料博览, 2003(11):20
[4] Chang B Y, Song R, Ding Y S, et al. China Feed, 2002(9):23常碧影, 宋荣, 丁永胜, 等. 中国饲料, 2002(9):23
[5] Huang T, Tao B H, Chen Q. et al. Journal of Food Safety and Quality, 2014, 5(7):2059黄焘, 陶保华, 陈启, 等. 食品安全质量检测学报, 2014, 5(7):2059
[6] Ding Y S, Mou S F. Chinese Journal of Chromatography, 2004, 22(2):174丁永胜, 牟世芬. 色谱, 2004, 22(2):174
[7] Ma P Y, Zhang Y L, Du S. Science and Technology Economic Guide, 2016(6):121马骋远, 张宇丽, 杜爽. 科技经济导刊, 2016(6):121
[8] GB/T 17481-2008
[9] Liu M X, Hu C X, Ren L, et al. Journal of Food Safety and Quality, 2013, 4(6):1880刘美霞, 胡彩霞, 任丽, 等. 食品安全质量检测学报, 2013, 4(6):1880
[10] Lin L, Wang H B. Chinese Journal of Health Laboratory Technology, 2013, 23(7):1706林立, 王海波. 中国卫生检验杂志, 2013, 23(7):1706
[11] Hu J Z, Wei H, Ren H. China Feed, 2003(20):19胡京枝, 魏红, 任红. 中国饲料, 2003(20):19
[12] Zhang H M. Cereal and Feed Industry, 2015(4):68张红梅. 粮食与饲料工业, 2015(4):68
[13] Liu Y M, Li G F, Fan X M, et al. Jiangxi Feed, 2002(6):21刘耀敏, 李国富, 范小敏, 等. 江西饲料, 2002(6):21
[14] Yang H Y. Tianjin Agricultural Sciences, 2010, 16(2):72杨鸿雁. 天津农业科学, 2010, 16(2):72
[15] Liu X M, Liu B, Guo Y, et al. Chinese Journal of Food Hygiene, 2016, 28(2):219刘雪梅, 刘波, 郭园, 等. 中国食品卫生杂志, 2016, 28(2):219
[16] Wang J J, Zhang W, Tan B Y. et al. Acta Nutrimenta Sinica, 2011, 33(6):607王佳佳, 张伟, 谭炳炎, 等. 营养学报, 2011, 33(6):607
[17] Zhang Y F, Zhao S, Wang J. et al. Chinese Journal of Forensic Medicine, 2013, 28(6):475张云峰, 赵森, 王炯, 等. 中国法医学杂志, 2013, 28(6):475
[18] Matuszewski B K, Constanzer M L, Chavez-Eng C M. Anal Chem, 2003, 75:3019
[19] Tang C L, Wang L, Cheng M C, et al. Chinese Journal of Chromatography, 2015, 33(7):699唐春兰, 王莉, 程孟春, 等. 色谱, 2015, 33(7):699