Determination of Pb2+ in tea by bis(salicylaldehyde)-o-phenylenediamine pre-column derivatization-high performance liquid chromatography

doi:10.3724/SP.J.1123.2017.03045

Abstract

Abstract:

The contents of Pb²⁺ in tea was determined by the high performance liquid chromatography (HPLC) method using bis(salicylaldehyde)-o-phenylenediamine (SALOPHEN) as derivatization reagent. The detection wavelength of ultraviolet was 226 nm with Hypersil ODS2 C₁₈ column (250 mm×4.6 mm, 5 μ m) as stationary phase, and pH 10.0 methanol-water (80:20, v/v) as mobile phase. The linear range of Pb²⁺ was 0.1-30 mg/L and the linear correlation coefficient was 0.9988. The limit of detection was 0.01 mg/L with the recoveries of 91.87%-96.96%. The method has high sensitivity and stability with satisfactory results, which can be used for the detection of Pb²⁺ in tea samples.

Key words: bis(salicylaldehyde)-o-phenylenediamine, heavy metal ions, high performance liquid chromatography (HPLC), tea

CLC Number:

O658

LU Yuxi, LUAN Feng, LIU Huitao. Determination of Pb²⁺ in tea by bis(salicylaldehyde)-o-phenylenediamine pre-column derivatization-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2017, 35(8): 843-847.

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Determination of Pb²⁺ in tea by bis(salicylaldehyde)-o-phenylenediamine pre-column derivatization-high performance liquid chromatography

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