Abstract:

A high performance liquid chromatographic (HPLC) method with gradient elution was developed for the simultaneous determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus. The analytes were separated on a Waters 5C18-MS-Ⅱcolumn (4.6 mm×250 mm) and detected at 220 nm. The mobile phases were methanol-1%(v/v) diethylamine solution (adjusted to pH 7.3 with phosphate) for gradient elution at temperature of 25 ℃. The calibration curves for vindoline, catharanthine and anhydrovinblastine showed good linearity in the ranges of 0.03-1 mg/mL (r=0.9997), 0.03-1 mg/mL (r=0.9999) and 0.01-0.5 mg/mL (r=0.9986), respectively. The recoveries were 96.8%, 97.0%, and 96.4% and the relative standard deviations (RSDs) were 1.53%, 1.37%, and 1.96%. The method is accurate, rapid and simple for the determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus.

Key words: anhydrovinblastine, catharanthine, Catharanthus roseus , vindoline, high performance liquid chromatography (HPLC)