Simultaneous determination of fifteen ultraviolet filters in sunscreen cosmetics by high performance liquid chromatography

doi:10.3724/SP.J.1123.2015.03024

Abstract

Abstract:

An analytical method for the simultaneous determination of fifteen ultraviolet (UV) filters in sunscreen cosmetics was developed by high performance liquid chromatography (HPLC). Various cosmetic samples including lotions, emulsions, creams and waxes were firstly mixed thoroughly by adding tetrahydrofuran containing 2 g/L ammonium hydroxide followed by vortex and oscillation. If the wax samples were still homogenized incompletely, a ultrasonic oscillation protocol with heating to 50 ℃ was adopted. The homogeneous samples were then ultrasonically extracted by 80% aqueous methanol solution. After centrifugation and micropore filtration, the extracted components were separated on an XTerra MS C₁₈ column with gradient elution by water containing 0.1%(v/v) formic acid and methanol containing 0.1%(v/v) formic acid, determined by a diode array detector (DAD) at 280 nm and 311 nm, and quantified using external standard method. Optimization for the pretreatment conditions of different matrices of cosmetics including sample dispersion solvents, extraction solvents and time were carried out. This developed method showed good linearity (r²≥0.9991) and the limits of quantification (LOQs) were between 1.2 and 5.1 μg/g. The recoveries of the fifteen compounds ranged from 84.2% to 100.7% at three spiked levels with the relative standard deviations (RSDs) between 0.9% and 9.5%. The analytes were well separated and this analytical method proved to be sensitive and accurate, so it can be used in the practical determination of the fifteen UV filters in commercial sunscreen cosmetics.

Key words: high performance liquid chromatography (HPLC), sunscreen cosmetics, ultraviolet (UV) filters

CLC Number:

O658

MENG Xianshuang, MA Qiang, BAI Hua, ZHANG Qing, LÜ Qing. Simultaneous determination of fifteen ultraviolet filters in sunscreen cosmetics by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2015, 33(8): 799-804.

References

[1] Lin W X, Sun X Q, Ma J. Chinese Journal of Chromatography (林维宣, 孙兴权, 马杰. 色谱), 2013, 31(5): 410

[2] He Q S, Xu N, Li J, et al. Chinese Journal of Chromatography (何乔桑, 徐娜, 李晶, 等. 色谱), 2011, 29(8): 762

[3] Wang R, Tian F Z. Advances Materials Industry (王锐, 田福祯. 新材料产业), 2012(7): 58

[4] Murong X. Detergent & Cosmetics (慕容秀. 日用化学品科学), 2008, 31(7): 4

[5] Zheng L, Yu W J, Shen J. China Foreign Medical Treatment (郑利, 余雯静, 沈洁. 中外医疗), 2009, 28(10): 120

[6] Cao J F, Zhou J. China Surfactant Detergent & Cosmetics (曹锦芳, 周洁. 日用化学工业), 2004, 34(4): 259

[7] Azurdia R M, Pagliaro J A, Rhodes L E. Photodermatol Photo, 2000, 16(2): 53

[8] Ministry of Health of the People's Republic of China. Hygienic Standard for Cosmetics. Beijing: Ministry of Health of the People's Republic of China (中华人民共和国卫生部. 化妆品卫生规范. 北京: 中华人民共和国卫生部), 2007

[9] Zhang G F, Mortier K A, Storozhenko S, et al. Rapid Commun Mass Spectrom, 2005, 19(8): 963

[10] Meinerling M, Daniels M. Anal Bioanal Chem, 2006, 386(5): 1465

[11] Rodil R, Quintana J B, López-Mahía P, et al. Anal Chem, 2008, 80(4): 1307

[12] Rodil R, Quintana J B, López-Mahía P, et al. J Chromatogr A, 1216: 2958

[13] Mao X Q, Bian H T, Qu B C. Chinese Journal of Chromatography (毛希琴, 边海涛, 曲宝成. 色谱), 2013, 31(8): 775

[14] Wang Y, Li X S. Flavor Fragrance Cosmetics (王晔, 李祥胜. 香料香精化妆品), 2014(4): 49

[15] Xie Q Y, Hou X F. China Pharmacist (谢琼玉, 侯晓斐. 中国药师), 2014, 17(11): 1977

[16] Guo C H, Zhang K D, Cai Y T, et al. Guangdong Chemical Industry (郭长虹, 张可冬, 蔡永通, 等. 广东化工), 2014, 41(15): 238

[17] Chen B, Li L, Ji W L. The Administration and Technique of Environment Monitoring (陈蓓, 李莉, 吉文亮. 环境监测管理与技术), 2010, 22(6): 61

[18] Zhang K D, Li H Y, Tan J H, et al. Chinese Journal of Analysis Laboratory (张可冬, 李慧勇, 谭建华, 等. 分析试验室), 2014, 33(9): 1108

[19] Gao L X, Zhang W Q, Yu X Y. Modern Preventive Medicine (高立雪, 张卫强, 喻晓毅. 现代预防医学), 2011, 38(7): 1324

[20] Xu X M, Gao Y H, Long C Y, et al. Chinese Journal of Health Laboratory Technology (许秀敏, 高燕红, 龙朝阳, 等. 中国卫生检验杂志), 2011, 21(7): 1601