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铋基金属有机骨架的合成与过氧化氢即时检测--本科生创新实验教学改革与实践

林宇乐1, 金成成1,2, 田苗苗1,*   

  1. 1.哈尔滨师范大学化学化工学院, 黑龙江 哈尔滨 150025;
    2.同济大学化学科学与工程学院, 上海 200092
  • 收稿日期:2026-03-19
  • 通讯作者: *Tel： 0451-88060570， E-mail： mmttqqq@163.com.
  • 基金资助:
    2025黑龙江省大学生创新创业训练项目（S202510231091）.

Teaching reform and practice of undergraduate innovation experiment based on synthesis of bismuth-based metal-organic frameworks and point-of-care testing of hydrogen peroxide

LIN Yule1, JIN Chengcheng1,2, TIAN Miaomiao1,*   

  1. 1. College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin 150025;
    2. College of Chemical Science and Engineering, Tongji University, Shanghai 200092
  • Received:2026-03-19
  • Supported by:
    2025 Heilongjiang Province College Students' Innovation and Entrepreneurship Training Program （No. S202510231091）.

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摘要： 针对当前高校本科生化学实验教学中理论与实践衔接不紧密、创新方法与前沿技术融入不足的痛点,本论文结合前沿科技成果金属有机骨架（MOFs）的制备与光谱分析仪器的使用,设计了一套面向本科生的创新实验教学方案。该实验以铋基金属有机骨架的制备为核心,首先通过两步溶剂热法合成氨基修饰的铋基对苯二甲酸金属有机骨架（Bi-BDC-NH2）,再利用静电自组装法使金纳米颗粒（Au NPs）与Bi-BDC-NH2结合得到所需材料Bi-BDC-NH2@Au。并利用傅里叶变换红外光谱仪（FT-IR）对材料的官能团进行表征,3400 cm-1处的吸收峰表明材料中存在氨基,1550 cm-1和1380 cm-1处出现的羧基配位峰证实了Bi-BDC-NH2@Au的成功合成。通过扫描电子显微镜（SEM）对其进行形貌分析,结果显示Au NPs均匀负载于MOF表面,说明该材料具有良好的组装效果。最后,利用智能手机比色法和紫外分光光度计（UV-Vis）实现对过氧化氢（H2O2）的可视化定量即时检测（POCT）,线性范围为1~100 μmol/L,检出限为0.98 μmol/L。学生全程参与从材料设计、合成优化到性能评价的完整科研链条,既掌握了材料制备的基本思路,又提升了仪器操作与数据分析能力。本实验不仅强化学生对材料化学、分析化学与生物传感等多学科的认知,更以前沿成果为牵引,构建了“基础理论-实践操作-创新应用”的一体化培养路径,有效提升了学生的科学研究素养与创新思维,为高校创新型化学人才培养提供了可推广的实践范式。

关键词: 本科生创新实验, 金属有机骨架, 分析化学, 即时检测, 教学改革

Abstract: To address the deficiencies in undergraduate chemistry laboratory teaching, where the connection between theory and practice remains insufficiently close and the integration of innovative methods and frontier technologies is inadequate, this paper designs an innovative experimental teaching scheme for undergraduates by combining the preparation of metal-organic frameworks （MOFs）, a frontier scientific and technological achievement, with the use of spectroscopic analytical instruments. Centering on the preparation of a bismuth-based MOF, the experiment first synthesizes an amino-modified bismuth terephthalate MOF （Bi-BDC-NH2） via a solvothermal method, and then combines Au nanoparticles （Au NPs） with Bi-BDC-NH2 through electrostatic self-assembly to obtain the target material Bi-BDC-NH2@Au. Fourier transform infrared spectroscopy （FT-IR） was used to characterize the functional groups of the material. The absorption band at 3400 cm-1 indicates the presence of amino groups, and the carboxyl coordination bands at 1550 cm-1 and 1380 cm-1 confirm the successful synthesis of Bi-BDC-NH2@Au. Scanning electron microscopy （SEM） was employed for morphological analysis, showing that Au NPs are uniformly loaded on the MOF surface, which demonstrates the good assembly effect of the material. Finally, colorimetric detection combined with ultraviolet-visible spectrophotometry （UV-Vis） enables visual quantitative point-of-care testing （POCT） of hydrogen peroxide （H2O2）, with a linear range of 1-100 μmol/L and a limit of detection of 0.98 μmol/L. Students participate in the complete research chain from material design and synthesis optimization to performance evaluation, thereby acquiring the basic ideas of material preparation while improving their skills in instrument operation and data analysis. This experiment not only strengthens students' multidisciplinary understanding of materials chemistry, analytical chemistry, and biosensing, but also, with frontier achievements serving as the driving force, establishes an integrated cultivation pathway of “basic theory-practical operation-innovative application”, which effectively enhances students' scientific research literacy and innovative thinking and provides a replicable practical paradigm for cultivating innovative chemistry talents in universities.

Key words: undergraduate innovative experiment, metal-organic framework （MOF）, analytical chemistry, point-of-care testing （POCT）, teaching reform

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