Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2011, Vol. 29 ›› Issue (12): 1222-1229.DOI: 10.3724/SP.J.1123.2011.01222

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Preparation of chiral monolithic column with covalently bonded cellulose and their application to rapid enantioseparation

WANG Jiabin1, WANG Xiao2, LI Jianhua1, L Haixia3, LIN Xucong2, XIE Zenghong2, ZHANG Qiqing1*   

  1. 1. Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; 2. Institute of Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou 350108, China; 3. College of Materials Science and Engineering, Fuzhou University, Fuzhou 350108, China
  • Received:2011-07-06 Revised:2011-08-10 Online:2011-12-28 Published:2012-01-15

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Abstract: A chiral monolithic capillary column for rapid enantioseparation was prepared by covalently bonding of cellulose tris(4-methylbenzoate) (CTMB) on N-acryloxysuccinimide-based monolith. The preparation and derivatization conditions of the monolithic column were optimized. The successful grafting of CTMB was confirmed on the characterizations of the infrared spectrum and the cathodic electroosmotic flow (EOF). The effects of acetic acid concentration and methanol content on the enantioseparation were studied. The solvent resistance, reproducibility and stability of the monolithic column have also been investigated. The rapid enantioseparation of the five solutes (phenylalanine, tyrosine, tryptophan, propranolol and phenylethanol) with resolution (Rs) values up to 1.31 was achieved within 1.2 min on the prepared chiral capillary monolithic column by capillary electrochromatography.

Key words: chiral monolithic capillary column, covalent bonding, rapid enantioseparation, capillary electrochromatography (CEC), cellulose tris(4-methylbenzoate)