Determination of peptide antibiotics in animal food by capillary electrochromatography coupled with laser induced fluorescence

doi:10.3724/SP.J.1123.2017.12001

Abstract

Abstract:

A rapid and sensitive method was established for the analysis of peptide antibiotics (bacitracin, polymyxin B and colistin) in animal food by capillary electrochromatography (CEC) coupled with laser induced fluorescence (LIF) using 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as fluorogenic reagent. Peptide antibiotics were derivatized by NBD-F in 50 mmol/L borate buffer (pH 7.5) for 45 min at 60℃. The fluorescence derivatives of peptide antibiotics were separated on a packed phenyl capillary column with a mobile phase consisting of acetonitrile-potassium phosphate (pH 5.0, 10 mmol/L) (55:45, v/v) at the flow rate of 0.02 mL/min. A supplementary pressure of 3.8 MPa and a separation voltage of -10 kV were applied. The limits of detection (LODs, S/N=3) were 5.0-10.0 ng/mL, which fulfilled the requirement of maximum residue limits for examined peptide antibiotics. The method was applied to detect peptide antibiotics in milk and feed stuffs. The spiked recoveries of the three peptide antibiotics were 72.9%-112.4%. The method shows some advantages on the simpler pretreatment and higher sensitivity, which can be of great benefit to the residual analysis of the veterinary drugs.

Key words: 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), capillary electrochromatography (CEC), fluorescence derivatization, laser induced fluorescence (LIF) detection, peptide antibiotics (PTs)

CLC Number:

O658

LEI Xiaoyun, SONG Yunping, LI Xinuo, ZHANG Bingyu, WU Xiaoping. Determination of peptide antibiotics in animal food by capillary electrochromatography coupled with laser induced fluorescence[J]. Chinese Journal of Chromatography, 2018, 36(3): 309-316.

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