Simultaneous determination of twenty psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2014.03002

Abstract

Abstract:

A method for the simultaneous determination of 20 psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) was established. The samples were extracted with alkalified acetonitrile, and then cleaned up using solid phase extraction with an Oasis MCX column. The UPLC separation was performed on a C18 column (50 mm×2.1 mm, 1.7 μm) using a gradient elution with the mobile phases of 0.1% (v/v) formic acid in water and acetonitrile. The ESI-MS/MS detection was achieved in positive mode under multiple reaction monitoring (MRM) mode. The calibration curves showed good linearity in the range of 5-100 μg/L with the correlation coefficients more than 0.99 for the 20 psychoactive drugs. The limits of quantification (LOQ, S/N≥10) for the 20 psychoactive drugs were 5 μg/kg. The average recoveries of the 20 psychoactive drugs spiked in blank pork at three levels of 5, 10 and 50 μg/kg were 66.8%-97.2% with the relative standard deviations from 4.2% to 12.4%. The method is suitable for the simultaneous determination of the 20 psychoactive drugs in pork with the characteristics of easy operation, high accuracy and precision.

Key words: pork, psychoactive drugs, solid phase extraction (SPE), ultra performance liquid chromatography-electrospary ionization tandem mass spectrometry (UPLC-ESI-MS/MS)

CLC Number:

O658

SUN Ting, WANG Luxiao, ZENG Sanmei, WU Min, YAN Lijuan, ZHANG Feng, HUANG Zhiqiang. Simultaneous determination of twenty psychoactive drugs in pork by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(7): 702-706.

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