Chinese Journal of Chromatography ›› 2021, Vol. 39 ›› Issue (5): 472-477.DOI: 10.3724/SP.J.1123.2020.07008
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QU Li1,2,*(), GU Shuqing1, ZHANG Jiaqi1, ZHAO Chaomin1, DENG Xiaojun1
Received:
2020-07-16
Online:
2021-05-08
Published:
2021-03-31
Contact:
QU Li
Supported by:
CLC Number:
QU Li, GU Shuqing, ZHANG Jiaqi, ZHAO Chaomin, DENG Xiaojun. Determination of 18 amino acids in three different kinds of milk powder by ultra performance liquid chromatography coupled with pre-column derivatization[J]. Chinese Journal of Chromatography, 2021, 39(5): 472-477.
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URL: https://www.chrom-china.com/EN/10.3724/SP.J.1123.2020.07008
Compound | Mr | Linear range/(nmol/mL) | Linear equation | r2 | LOD/(mg/100 g) | LOQ/(mg/100 g) |
---|---|---|---|---|---|---|
His | 155.16 | 0-20 | Y=7.65×103X-5.77×102 | 0.9998 | 2.5 | 7.5 |
Tau | 125.15 | 0-20 | Y=8.31×103X-1.78×102 | 0.9999 | 2.4 | 7.2 |
Ser | 105.09 | 0-20 | Y=7.64×103X+8.15×102 | 0.9998 | 2.2 | 6.6 |
Arg | 174.20 | 0-20 | Y=7.56×103X+1.17×103 | 0.9997 | 2.5 | 7.5 |
Gly | 75.07 | 0-20 | Y=7.54×103X+4.50×102 | 0.9998 | 2.1 | 6.3 |
Asp | 133.11 | 0-20 | Y=7.68×103X+5.14×102 | 0.9998 | 2.2 | 6.6 |
Glu | 147.13 | 0-20 | Y=7.22×103X+2.28×103 | 0.9997 | 1.6 | 5.0 |
Thr | 119.12 | 0-20 | Y=7.90×103X+8.52×102 | 0.9998 | 1.4 | 4.5 |
Ala | 89.10 | 0-20 | Y=7.84×103X+1.61×103 | 0.9998 | 1.3 | 3.9 |
Pro | 115.13 | 0-20 | Y=7.34×103X+2.10×103 | 0.9997 | 2.1 | 6.3 |
Lys | 146.19 | 0-20 | Y=1.34×104X+1.66×102 | 0.9997 | 1.3 | 3.9 |
Tyr | 181.19 | 0-20 | Y=8.20×103X+2.90×103 | 0.9996 | 2.1 | 6.3 |
Met | 149.21 | 0-20 | Y=8.07×103X+1.91×103 | 0.9998 | 1.7 | 5.1 |
Val | 117.15 | 0-20 | Y=8.21×103X-1.34×102 | 0.9996 | 1.3 | 4.0 |
Iso | 131.18 | 0-20 | Y=8.13×103X+1.62×103 | 0.9997 | 2.2 | 4.5 |
Leu | 131.18 | 0-20 | Y=8.23×103X+2.05×103 | 0.9997 | 2.3 | 6.9 |
Phe | 165.19 | 0-20 | Y=8.09×103X+1.12×103 | 0.9998 | 2.1 | 4.2 |
xCys | 240.30 | 0-10 | Y=1.31×104X-2.09×102 | 0.9999 | 2.5 | 7.5 |
Table 1 Mr, linear equations, correlation coefficients (r2), LODs and LOQs of the 18 amino acids
Compound | Mr | Linear range/(nmol/mL) | Linear equation | r2 | LOD/(mg/100 g) | LOQ/(mg/100 g) |
---|---|---|---|---|---|---|
His | 155.16 | 0-20 | Y=7.65×103X-5.77×102 | 0.9998 | 2.5 | 7.5 |
Tau | 125.15 | 0-20 | Y=8.31×103X-1.78×102 | 0.9999 | 2.4 | 7.2 |
Ser | 105.09 | 0-20 | Y=7.64×103X+8.15×102 | 0.9998 | 2.2 | 6.6 |
Arg | 174.20 | 0-20 | Y=7.56×103X+1.17×103 | 0.9997 | 2.5 | 7.5 |
Gly | 75.07 | 0-20 | Y=7.54×103X+4.50×102 | 0.9998 | 2.1 | 6.3 |
Asp | 133.11 | 0-20 | Y=7.68×103X+5.14×102 | 0.9998 | 2.2 | 6.6 |
Glu | 147.13 | 0-20 | Y=7.22×103X+2.28×103 | 0.9997 | 1.6 | 5.0 |
Thr | 119.12 | 0-20 | Y=7.90×103X+8.52×102 | 0.9998 | 1.4 | 4.5 |
Ala | 89.10 | 0-20 | Y=7.84×103X+1.61×103 | 0.9998 | 1.3 | 3.9 |
Pro | 115.13 | 0-20 | Y=7.34×103X+2.10×103 | 0.9997 | 2.1 | 6.3 |
Lys | 146.19 | 0-20 | Y=1.34×104X+1.66×102 | 0.9997 | 1.3 | 3.9 |
Tyr | 181.19 | 0-20 | Y=8.20×103X+2.90×103 | 0.9996 | 2.1 | 6.3 |
Met | 149.21 | 0-20 | Y=8.07×103X+1.91×103 | 0.9998 | 1.7 | 5.1 |
Val | 117.15 | 0-20 | Y=8.21×103X-1.34×102 | 0.9996 | 1.3 | 4.0 |
Iso | 131.18 | 0-20 | Y=8.13×103X+1.62×103 | 0.9997 | 2.2 | 4.5 |
Leu | 131.18 | 0-20 | Y=8.23×103X+2.05×103 | 0.9997 | 2.3 | 6.9 |
Phe | 165.19 | 0-20 | Y=8.09×103X+1.12×103 | 0.9998 | 2.1 | 4.2 |
xCys | 240.30 | 0-10 | Y=1.31×104X-2.09×102 | 0.9999 | 2.5 | 7.5 |
Compound | Milk powder of the same brand at different stages | Different powder of the same brand at stage 1 | Different brands of milk powder of goat | Different origins of milk powder of camel | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Stage 1 | Stage 2 | Stage 3 | Milk powder | Goat powder | Brand 1 | Brand 2 | Brand 3 | Xinjiang 1 | Xinjiang 2 | Dubai | |||||
His* | 1.97 | 2.14 | 2.25 | 1.94 | 2.40 | 2.49 | 2.44 | 2.49 | 2.25 | 2.28 | 2.49 | ||||
Tau | 0.39 | 0.28 | 0.25 | 0.31 | 0.17 | 0.17 | 0.21 | / | 0.21 | 0.21 | / | ||||
Ser | 4.74 | 5.11 | 5.23 | 5.11 | 5.22 | 5.10 | 5.04 | 5.06 | 5.21 | 5.25 | 5.03 | ||||
Arg | 2.74 | 2.90 | 2.98 | 2.35 | 2.64 | 2.45 | 2.47 | 2.84 | 3.03 | 2.95 | 3.89 | ||||
Gly | 2.43 | 1.89 | 1.87 | 1.68 | 1.64 | 1.63 | 1.69 | 1.75 | 1.87 | 1.80 | 1.44 | ||||
Asp | 9.11 | 8.72 | 7.94 | 9.13 | 7.92 | 7.90 | 7.65 | 7.33 | 8.34 | 8.31 | 7.08 | ||||
Glu | 19.0 | 20.1 | 21.0 | 20.2 | 21.2 | 20.9 | 20.9 | 22.0 | 20.7 | 20.7 | 21.8 | ||||
Thr* | 5.10 | 5.26 | 5.03 | 5.46 | 5.43 | 5.37 | 5.25 | 4.64 | 4.90 | 4.92 | 4.80 | ||||
Ala | 4.42 | 4.02 | 3.88 | 4.19 | 3.75 | 3.79 | 3.62 | 3.13 | 3.76 | 3.72 | 2.50 | ||||
Pro | 6.93 | 8.03 | 8.52 | 7.70 | 9.98 | 9.85 | 9.89 | 10.1 | 8.40 | 8.59 | 10.3 | ||||
Lys* | 8.93 | 8.54 | 8.62 | 8.30 | 7.60 | 8.15 | 8.12 | 7.92 | 8.23 | 8.15 | 7.41 | ||||
Tyr | 3.53 | 3.93 | 4.52 | 3.52 | 3.11 | 3.83 | 4.00 | 4.23 | 4.43 | 4.32 | 4.57 | ||||
Met* | 2.25 | 2.34 | 1.27 | 2.26 | 2.16 | 2.28 | 2.45 | 2.50 | 2.49 | 2.51 | 2.84 | ||||
Val* | 6.01 | 5.94 | 6.06 | 6.14 | 6.79 | 6.67 | 6.87 | 6.56 | 6.03 | 6.12 | 6.21 | ||||
Iso* | 5.37 | 5.49 | 5.38 | 5.72 | 5.05 | 4.92 | 4.84 | 4.65 | 5.25 | 5.26 | 5.22 | ||||
Leu* | 10.6 | 9.66 | 9.58 | 9.98 | 9.18 | 8.97 | 9.02 | 9.20 | 9.41 | 9.44 | 9.21 | ||||
Phe* | 3.84 | 4.17 | 4.33 | 4.11 | 4.57 | 4.40 | 4.42 | 4.73 | 4.28 | 4.21 | 4.38 | ||||
xCys | 2.67 | 1.54 | 1.29 | 1.86 | 1.25 | 1.15 | 1.13 | 0.90 | 1.23 | 1.29 | 0.78 | ||||
TAA/(mg/100 g) | 11731 | 17116.5 | 19584 | 12777 | 15698 | 16029 | 16452 | 28806 | 20531 | 20108 | 24073 | ||||
TEAA/(mg/100 g) | 4716 | 6738 | 7480 | 5087 | 6060 | 6225 | 6415 | 10937 | 7917 | 7777 | 9190 | ||||
TEAA in TAA/% | 40.2 | 39.4 | 38.2 | 39.8 | 38.6 | 38.8 | 39.0 | 38.0 | 38.6 | 38.7 | 38.2 |
Table 2 Percentages of the 18 AAs in total amino acids (TAAs) in the different stages of milk powder with the same brand, cow and goat milk powder of the same brand at the same stage, goat milk powder of the three different brands, and camel milk powder of different origins %
Compound | Milk powder of the same brand at different stages | Different powder of the same brand at stage 1 | Different brands of milk powder of goat | Different origins of milk powder of camel | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Stage 1 | Stage 2 | Stage 3 | Milk powder | Goat powder | Brand 1 | Brand 2 | Brand 3 | Xinjiang 1 | Xinjiang 2 | Dubai | |||||
His* | 1.97 | 2.14 | 2.25 | 1.94 | 2.40 | 2.49 | 2.44 | 2.49 | 2.25 | 2.28 | 2.49 | ||||
Tau | 0.39 | 0.28 | 0.25 | 0.31 | 0.17 | 0.17 | 0.21 | / | 0.21 | 0.21 | / | ||||
Ser | 4.74 | 5.11 | 5.23 | 5.11 | 5.22 | 5.10 | 5.04 | 5.06 | 5.21 | 5.25 | 5.03 | ||||
Arg | 2.74 | 2.90 | 2.98 | 2.35 | 2.64 | 2.45 | 2.47 | 2.84 | 3.03 | 2.95 | 3.89 | ||||
Gly | 2.43 | 1.89 | 1.87 | 1.68 | 1.64 | 1.63 | 1.69 | 1.75 | 1.87 | 1.80 | 1.44 | ||||
Asp | 9.11 | 8.72 | 7.94 | 9.13 | 7.92 | 7.90 | 7.65 | 7.33 | 8.34 | 8.31 | 7.08 | ||||
Glu | 19.0 | 20.1 | 21.0 | 20.2 | 21.2 | 20.9 | 20.9 | 22.0 | 20.7 | 20.7 | 21.8 | ||||
Thr* | 5.10 | 5.26 | 5.03 | 5.46 | 5.43 | 5.37 | 5.25 | 4.64 | 4.90 | 4.92 | 4.80 | ||||
Ala | 4.42 | 4.02 | 3.88 | 4.19 | 3.75 | 3.79 | 3.62 | 3.13 | 3.76 | 3.72 | 2.50 | ||||
Pro | 6.93 | 8.03 | 8.52 | 7.70 | 9.98 | 9.85 | 9.89 | 10.1 | 8.40 | 8.59 | 10.3 | ||||
Lys* | 8.93 | 8.54 | 8.62 | 8.30 | 7.60 | 8.15 | 8.12 | 7.92 | 8.23 | 8.15 | 7.41 | ||||
Tyr | 3.53 | 3.93 | 4.52 | 3.52 | 3.11 | 3.83 | 4.00 | 4.23 | 4.43 | 4.32 | 4.57 | ||||
Met* | 2.25 | 2.34 | 1.27 | 2.26 | 2.16 | 2.28 | 2.45 | 2.50 | 2.49 | 2.51 | 2.84 | ||||
Val* | 6.01 | 5.94 | 6.06 | 6.14 | 6.79 | 6.67 | 6.87 | 6.56 | 6.03 | 6.12 | 6.21 | ||||
Iso* | 5.37 | 5.49 | 5.38 | 5.72 | 5.05 | 4.92 | 4.84 | 4.65 | 5.25 | 5.26 | 5.22 | ||||
Leu* | 10.6 | 9.66 | 9.58 | 9.98 | 9.18 | 8.97 | 9.02 | 9.20 | 9.41 | 9.44 | 9.21 | ||||
Phe* | 3.84 | 4.17 | 4.33 | 4.11 | 4.57 | 4.40 | 4.42 | 4.73 | 4.28 | 4.21 | 4.38 | ||||
xCys | 2.67 | 1.54 | 1.29 | 1.86 | 1.25 | 1.15 | 1.13 | 0.90 | 1.23 | 1.29 | 0.78 | ||||
TAA/(mg/100 g) | 11731 | 17116.5 | 19584 | 12777 | 15698 | 16029 | 16452 | 28806 | 20531 | 20108 | 24073 | ||||
TEAA/(mg/100 g) | 4716 | 6738 | 7480 | 5087 | 6060 | 6225 | 6415 | 10937 | 7917 | 7777 | 9190 | ||||
TEAA in TAA/% | 40.2 | 39.4 | 38.2 | 39.8 | 38.6 | 38.8 | 39.0 | 38.0 | 38.6 | 38.7 | 38.2 |
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