Chinese Journal of Chromatography

Chinese Journal of Chromatography ›› 2015, Vol. 33 ›› Issue (10): 1104-1109.DOI: 10.3724/SP.J.1123.2015.04048

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Rapid screening and identification of 22 allergenic disperse dyes in ecological textiles by high performance liquid chromatography- linear ion trap/orbitrap mass spectrometry

NIU Zengyuan1, LUO Xin1, YE Xiwen1, XIU Xiaoli1,2, ZHANG Li1,3, WANG Xin1, CHEN Jing1   

  1. 1. Technical Center for Inspection and Quarantine of Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China;
    2. Collage of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;
    3. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
  • Received:2015-04-28 Online:2015-10-08 Published:2012-05-09

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Abstract:

A rapid screening method based on high performance liquid chromatography-linear ion trap/orbitrap high-resolution mass spectrometry (HPLC-LTQ/Orbitrap MS) for 22 disperse dyes in ecological textiles has been established. The target compounds were extracted by pyridine/water (1:1, v/v) by shaking extraction in 90 ℃ water bath. The extracts were then separated by a CAPCELL PAK C18 column (100 mm×2.0 mm, 5 μm) using gradient elution with acetonitrile-5 mmol/L ammonium acetate containing 0.01% (v/v) formic acid as mobile phases, and finally analyzed by HPLC-LTQ/Orbitrap in positive and negative ESI modes. The retention time and accurate mass of parent ion were used for fast screening of 22 disperse dyes, while the confirmatory analysis was obtained by fragments generated by collision-induced dissociation (CID) MS/MS. Target analysis exhibited high mass accuracy (< 5×10-6). Each target showed a good linearity in its own concentration range and the correlation coefficient was higher than 0.99. The LOQs were 0.125-2.5 mg/kg. Except for Disperse Yellow 49, the average recoveries of most disperse dyes at three spiked levels were 65%-120%, and the relative standard deviations (n=6) were less than 15%. The method was applied for screening 40 different kinds of textiles, and Disperse Orange 37/76 was detected in one of them. With high selectivity and strong anti-jamming ability, this method is simple, rapid, accurate, and it can be used for the inspection of disperse dyes in textiles.

Key words: disperse dyes, high performance liquid chromatography-linear ion trap/orbitrap mass spectrometry (HPLC-LTQ/Orbitrap MS), high resolution mass spectrometry (HRMS), textiles

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