Determination of ginkgolic acids in the ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry

doi:10.3724/SP.J.1123.2015.08027

Abstract

Abstract:

An analytical method was developed for the determination of ginkgolic acids in ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry. A chromatographic column, Agilent Poroshell 120 EC-C18(50 mm×3.0 mm, 2.7 μ m), was used with methanol-1% acetic acid (90 : 10, v/v) as the mobile phase. The ginkgo acids were detected by electrospray ionization mass spectrometry in negative mode with multiple reaction monitoring (MRM) mode. Ginkgo acids C13 : 0, C15 : 1 and C17 : 1 possessed good linear correlations in the mass concentration range from 2 to 200 μ g/L, with the correlation coefficients more than 0.999. The mean recoveries at the spiked levels of 5, 20 and 100 μ g/L were in the range of 86.3%-114.3%, and the RSDs were 0.5%-13.6%. The limits of detection and quantification were 0.003-0.08 μ g/g and 0.01-0.19 μ g/g, respectively. The method was successfully applied to the analysis of actual samples.

Key words: ginkgo biloba extract, ginkgo leaf capsule, ginkgo leaf tablet, ginkgolic acid, ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)

CLC Number:

O658

SUN Jian, LI Limin, HU Qing, MAO Xiuhong, JI Shen. Determination of ginkgolic acids in the ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(2): 184-188.

References

[1] Qi H Z, Zhou X J, Wang M X. Hebei Journal of Traditional Chinese Medicine, 2013, 35(12): 1899 齐惠珍, 周霞瑾, 王明霞. 河北中医, 2013, 35(12): 1899
[2] Liu P P, Pan S H. China Journal of Chinese Materia Medica, 2012, 37(3): 274 刘平平, 潘苏华. 中国中药杂志, 2012, 37(3): 274
[3] Wu X Y, Yang L Q, Chen J. Acta Pharmaceutica Sinica, 2003, 38(11): 846 吴向阳, 仰榴青, 陈钧. 药学学报, 2003, 38(11): 846
[4] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, Part 1. Beijing: China Medical Science Press, 2010: 392 国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社, 2010: 392
[5] European Pharmacopoeia 8.0: 1257
[6] U. S. Pharmacopoeia 38: 6064
[7] Nie L X, Lu J. Chinese Journal of Pharmaceutical Analysis, 2005, 25(8): 909 聂黎行, 鲁静. 药物分析杂志, 2005, 25(8): 909
[8] Yao J B, Fang L, Wang R W, et al. Chinese Journal of Pharmaceutical Analysis, 2012, 32(11): 2055 姚建标, 方玲, 王如伟, 等. 药物分析杂志, 2012, 32(11): 2055
[9] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, Part 1. Beijing: China Medical Science Press, 2015: 416 国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社, 2015: 416
[10] Li L C, Yin Z F, Yang Y Q, et al. China Practical Medicine, 2015, 10(1): 233 李兰崇, 尹志芳, 杨宇强, 等. 中国实用医药, 2015, 10(1): 233
[11] Zhang Z Y, Wang H N, Wang K Y, et al. Chinese Traditional Patent Medicine, 2012, 34(10): 2045 张志勇, 王海宁, 王克英, 等. 中成药, 2012, 34(10): 2045
[12] Yao X, Zhou G S, Tang Y P, et al. Biomed Res Int, 2013, 2013: 582591
[13] Wang L L, Jia Y L, Pan Z F, et al. Chinese Journal of Chromatography, 2008, 26(6): 731 王丽丽, 贾一律, 潘再法, 等. 色谱, 2008, 26(6): 731
[14] Yang X M, He J, Liu Y J, et al. Lishizhen Medicine and Materia Medica Research, 2009, 20(10): 2453 杨小明, 何军, 柳艳君, 等. 时珍国医国药, 2009, 20(10): 2453

[1]	DONG Jieqiong, XIAO Jin, ZHOU Xin, LI Ning, WANG Xuesong, KANG Junjie. Determination of 14 β-agonists in animal meat by ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2023, 41(12): 1106-1114.
[2]	SUN Huijing, LI Peiwen, ZHANG Beibei, CHEN Huiming. Determination of 42 antibiotic residues in seven categories in water using large volume direct injection by ultra high performance liquid chromatography-triple quadrupole mass spectrometry [J]. Chinese Journal of Chromatography, 2022, 40(4): 333-342.
[3]	SUN Qiang, LI Yubo, WEN Guangyue, WANG Weimin, DONG Maofeng, TANG Hongxia. Determination of disulfoton and its metabolites in agricultural products by dispersive soild phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2022, 40(2): 130-138.
[4]	CHEN Xinglian, LIN Tao, LIU Xingyong, MEI Wenquan, WANG Li, GENG Huichun, CHENG Long, WANG Luxiang. Rapid determination of multiple prohibited and restricted veterinary drug residues in fish and shrimp by ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2020, 38(5): 538-546.
[5]	XU Dunming, LAI Guoyin, CHEN Yan, LUO Chao, YI Xionghai, DENG Xiaojun, FENG Feng, ZHANG Feng. Simultaneous determination of 21 illegally added compounds in health foods by ultra high performance liquid chromatography-tandem mass spectrometry with solid phase extraction [J]. Chinese Journal of Chromatography, 2019, 37(7): 778-785.
[6]	ZHANG Xu, QIU Tian, FU Hui, YANG Yanwei, ZHAO Feng, LIN Shaobin, HU Xiaojian. Determination of nine phthalate ester metabolites in human urine using ultra high performance liquid chromatography-tandem triple quadrupole mass spectrometry [J]. Chinese Journal of Chromatography, 2018, 36(9): 895-903.
[7]	ZHOU Shaohuan, LIANG Chuan, TANG Yang, LIAO Yanhua, LIN Wensi. Determination of 15 functional components in sweet potato leaves by ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2018, 36(5): 425-430.
[8]	LING Aru, GUO Wenbo, YANG Junhua, ZHAO Zhihui, GUO Jinli. Simultaneous determination of six aflatoxins and sterigmatocystin in livestock and poultry tissues by QuEChERS-ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2018, 36(5): 439-445.
[9]	SUN Shanshan, ZHU Lijun, HU Yanxi, LIU Yufeng, XU Liang. Determination of sedative and β-receptor hormone residues in feed by ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2018, 36(2): 150-158.
[10]	HE Meilian, GUO Changchuan, LENG Jiawei, ZHANG Xunjie, XIAN Ruiqing, GONG Liping, SHI Feng, JIANG Wei. Determination of ambroxol hydrochloride in human plasma by ultra high performance liquid chromatography-tandem mass spectrometry and bioequivalence evaluation of its preparation [J]. Chinese Journal of Chromatography, 2018, 36(11): 1099-1104.
[11]	FAN Zhichen, HAN Zheng, GUO Wenbo, ZHAO Zhihui. Simultaneous determination of 30 mycotoxins in different feed products by ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2017, 35(6): 627-633.
[12]	LI Lingyun, XU Xiaomin, LIN Huan, ZHAO Wen, YE Rong, HUANG Xiaodong, ZHENG Shuning, LIU Xinyan, LIU Su, XU Donghui. Rapid detection of 248 pesticide residues in vegetables by ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2016, 34(9): 835-849.
[13]	WANG Xing, CHI Yumei, KANG An. Qualitative and quantitative analysis of the amino acids in Rhizoma Arisaematis by ultra high performance liquid chromatography-tandem mass spectrometry and high performance liquid chromatography [J]. Chinese Journal of Chromatography, 2014, 32(12): 1326-1332.
[14]	ZHAO Hui, WANG Yuhong, LIU Fang, WANG Yan, GU Xue, YAN Chao. Microfluidic evaporative light scattering detector coupled with capillary liquid chromatography and its application to Ginkgo biloba extract analysis [J]. Chinese Journal of Chromatography, 2013, 31(8): 795-799.
[15]	WEN Yuyun, OU Yan, HE Mingchao, GONG Zhenbin^*. Determination of carcinogenic aromatic amines derived from azo colorants in textiles and leather by ultra high performance liquid chromatography-tandem mass spectrometry [J]. Chinese Journal of Chromatography, 2013, 31(4): 380-385.