Determination of 23 antibiotics and 3 β-agonists in livestock drinking water by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid-phase extraction

doi:10.3724/SP.J.1123.2015.09021

Abstract

Abstract:

A method has been developed for the simultaneous determination of 23 antibiotics (four categories) and 3 β-agonists in livestock drinking water using solid-phase extraction and ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI MS/MS). The samples were adjusted pH to 5.0, added Na₂EDTA, enriched and cleaned-up by an HLB solid-phase extraction cartridge. The target compounds were confirmed and quantified by UPLC-ESI MS/MS with external standard method for the antibiotics and internal standard method for the β-agonists. The recoveries were assessed by using lab tap water as matrix. The average recoveries of the 23 antibiotics and the 3 β-agonists were in the range of 50.7%-104.6% and the relative standard deviations (RSDs) were 2.6%-8.8%(n=3). Under the optimal conditions, the calibration curves of the 23 antibiotics and the 3 β-agonists showed good linearity with the correlation coefficients better than 0.994. The limits of detection (LODs, S/N≥3) ranged from 0.01-0.20 ng/L. The developed method was applied to analyze the livestock drinking waters in 36 Beijing intensive livestock farms. The results showed that some antibiotics were detected.

Key words: β-agonists, antibiotics, livestock drinking water, solid-phase extraction, ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

CLC Number:

O658

SHI Ao. Determination of 23 antibiotics and 3 β-agonists in livestock drinking water by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid-phase extraction[J]. Chinese Journal of Chromatography, 2016, 34(2): 176-183.

References

[1] Qi Y J, Liu F. Rock and Mineral Analysis, 2014, 33(1): 1 祁彦洁, 刘菲. 岩矿测试, 2014, 33(1): 1
[2] Shi F Y, Guo X, Yang H C, et al. Chinese Journal of Veterinary Medicine, 2007, 43(8): 8 施奉英, 郭鑫, 杨汉春, 等. 中国兽医杂志, 2007, 43(8): 8
[3] He M, Yi B C, Chen M. Heilongjiang Animal Science and Veterinary Medicine, 2011(3): 27 何敏, 易本驰, 陈敏. 黑龙江畜牧兽医, 2011(3): 27
[4] Ye B X, Zhang L. Journal of Environment and Health, 2015, 32(2): 173 叶必雄, 张岚. 环境与健康杂志, 2015, 32(2): 173
[5] Hu X Y. Food and Drug, 2015, 17(3): 215 胡譞予. 食品与药品, 2015, 17(3): 215
[6] Gao L H, Shi Y L, Li W H, et al. Environmental Chemistry, 2013, 32(9): 1619 高立红, 史亚利, 厉文辉, 等. 环境化学, 2013, 32(9): 1619
[7] Zhang Q, Xin Q, Zhu J M, et al. Environmental Chemistry, 2014, 33(7): 1075 章强, 辛琦, 朱静敏, 等. 环境化学, 2014, 33(7): 1075
[8] Sarmah A K, Meyer M T, Boxall A B A. Chemosphere, 2006, 65(5): 725
[9] Guo X Y, Wang N, Xu J, et al. Environmental Science & Technology, 2014, 37(9): 76 郭欣研, 王娜, 许静, 等. 环境科学与技术, 2014, 37(9): 76
[10] Ministry of Agriculture. No. 176 Bulletin of the Ministry of Agriculture of the People's Republic of China. (2002-02-09) [2008-04-16]. http://www.docin.com/p-486570614.html 农业部. 中华人民共和国农业部公告第176号. (2002-02-09) [2008-04-16]. http://www.docin.com/p-486570614.html
[11] Zhou A X, Su X S, Gao S, et al. Chinese Journal of Analytical Chemistry, 2014, 42(3): 397 周爱霞, 苏小四, 高松, 等. 分析化学, 2014, 42(3): 397
[12] Li L, Liu F, Chen H H, et al. Chinese Journal of Chromatography, 2013, 31(6): 567 李璐, 刘菲, 陈鸿汉, 等. 色谱, 2013, 31(6): 567
[13] Wu C Q, Lei J M, Li Y L, et al. Chinese Journal of Chromatography, 2014, 32(12): 1362 吴翠琴, 雷金妹, 李韵灵, 等. 色谱, 2014, 32(12): 1362
[14] Wang S, Zhang X M, Zhang J, et al. Chinese Journal of Chromatography, 2015, 33(7): 730 王硕, 张向明, 张晶, 等. 色谱, 2015, 33(7): 730
[15] Tang C M, Huang Q X, Yu Y Y, et al. Journal of Instrumental Analysis, 2009, 28(8): 909 唐才明, 黄秋鑫, 余以义, 等. 分析测试学报, 2009, 28(8): 909
[16] Gao L H, Shi Y L, Li W F, et al. Chinese Journal of Chromatography, 2010, 28(5): 491 高立红, 史亚利, 厉文辉, 等. 色谱, 2010, 28(5): 491
[17] Sun G D, Su Z Y, Chen M, et al. Chinese Journal of Chromatography, 2009, 27(1): 54 孙广大, 苏仲毅, 陈猛, 等. 色谱, 2009, 27(1): 54
[18] Zhang X Y, Li Z Q, Li L C, et al. Journal of Zhongzhou University, 2013, 30(4): 114 张晓燕, 李志强, 李领川, 等. 中州大学学报, 2013, 30(4): 114
[19] Lu K X, Sui M H, Gao N Y. Journal of Instrumental Analysis, 2010, 29(12): 1209 陆克祥, 隋铭皓, 高乃云. 分析测试学报, 2010, 29(12): 1209
[20] 2002-657-EC
[21] Ardrey R E. Liquid Chromatography-Mass Spectrometry: An Introduction. Chichester, UK: John Wiley & Sons, Ltd., 2003
[22] GB/T 22338-2008