Enantioseparation of chiral pesticide flutriafol enantiomers on a methyl phenyl ureado β-cyclodextrin-bonded stationary phase by high performance liquid chromatography

doi:10.3724/SP.J.1123.2015.09017

Abstract

Abstract:

A new mono-methyl phenyl ureado β-cyclodextrin-bonded order mesoporous SBA-15 chiral stationary phase (UCDP) was prepared. The basic structure characterization of the new stationary phase was carried out by infrared spectroscopy and elemental analysis. The new packing material was used to separate chiral fungicide flutriafol enantiomers by HPLC. The effects of the composition of mobile phase and column temperature on the enantioseparation of flutriafol were investigated. The flutriafol enantiomers were successfully resolved under the optimized conditions, which were as follows: methanol-water (volume ratio of 40 : 60) as mobile phase at a flow rate of 0.5 mL/min, the column temperature at 25 ℃, the detection wavelength at 205 nm. The resolution of the two enantiomers was up to 1.99 within 20 min under the above conditions. Based on the study, a new method for the determination of flutriafol enantiomers in banana was established. Good linear relationships were observed in the mass concentration range of 0.5-125 mg/L (r=0.9997) for the two enantiomers. The average spiked recoveries were 93.4%-101.7% with RSDs of 1.07%-2.06% (n=4). The detection limit was 0.06 mg/L (S/N=3). Compared with the commonly used cellulose stationary phase, the preparation of new stationary phase is simple at low cost. The β-cyclodextrin-bonded stationary phase with mono-methyl phenyl ureado has high chemical stability, reproducible chromatographic property and high selectivity for flutriafol enantiomers. The developed analytical method is suitable for the fast monitoring of chiral pesticide residues in foods and has research significance to solve the food safety problems of chiral chemicals.

Key words: banana, flutriafol enantiomer, food safety, high performance liquid chromatography (HPLC), methyl phenyl ureado β-cyclodextrin-bonded phase

CLC Number:

O658

ZENG Chun, LI Laisheng, CHENG Biaoping, ZHANG Hongfu, CAO Zhigang, ZHOU Zhiyong. Enantioseparation of chiral pesticide flutriafol enantiomers on a methyl phenyl ureado β-cyclodextrin-bonded stationary phase by high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2016, 34(3): 314-320.

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