Metabolomics study in rat urine of adjuvant arthritis using gas chromatography-time-of-flight mass spectrometry

doi:10.3724/SP.J.1123.2016.01020

Abstract

Abstract:

Adjuvant arthritis (AA) rats induced by Freund's complete adjuvant (FCA) were used to observe the changes of the degree of swelling in the toes and pathomorphology of ankle joint tissue. Gas chromatography-time-of-flight mass spectrometry (GC-TOF MS) was employed to detect the metabolite spectrum in rat urine with AA, and the data were analyzed by principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), and to explore its possible pathogenesis. Several metabolites based on the variable importance in the projection values (VIP>1), P value (<0.05) and screening in databases were identified. The results showed that 20 potential biomarkers such as isocitric acid, α-ketoglutaric acid, citraconic acid, creatine and 3-hydroxybutyric acid which had a significant contribution to classification were selected. Comparing with the normal group, AA rats present an abnormal metabolism. This study illustrates that AA metabolomics pathogenesis may be related to regulating the dysfunction of energy metabolism, amino acid metabolism and fatty acid metabolism.

Key words: adjuvant arthritis (AA), gas chromatography-time-of-flight mass spectrometry (GC-TOF MS), metabolomics, urine

CLC Number:

O658

WANG Yongzhong, LIU Qing, JIANG Hui, HAN Yanquan, LI Yuxin. Metabolomics study in rat urine of adjuvant arthritis using gas chromatography-time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, 2016, 34(6): 602-607.

References

[1] Alghasham A, Rasheed Z. Autoimmunity, 2014, 47(2):77
[2] Li R, Meng Q G. Chinese Archives of Traditional Chinese Medicine, 2007, 25(7):1348 李瑞, 孟庆刚. 中华中医药学刊, 2007, 25(7):1348
[3] Ding X H, Hu J B, Li J F, et al. Anal Biochem, 2014, 458:49
[4] Wang F R, Wu Y, Ai Y, et al. Biomed Chromatogr, 2016, 30(3):396
[5] Lauridsen M B, Bliddal H, Christensen R, et al. J Proteome Res, 2010, 9(9):4545
[6] Deng L F, Wang Y Z, Han Y Q, et al. Chinese Journal of Cellular and Molecular Immunology, 2015, 31(2):153 邓龙飞, 汪永忠, 韩燕全, 等. 细胞与分子免疫学杂志, 2015, 31(2):153
[7] Zhang D.[MS Dissertation]. Shenyang:Shenyang Pharmaceutical University, 2010:269 张迪.[硕士学位论文]. 沈阳:沈阳药科大学, 2010:269
[8] Qi Y, Pi Z F, Liu S, et al. Mol Biosyst, 2014, 10(10):2617
[9] Terkeltaub R. Nat Rev Rheumatol, 2010, 6(1):30
[10] Zhang H W, Fu P, Ke B, et al. J Ethnopharmacol, 2014, 154(1):55
[11] Zabek A, Swierkot J, Malak A, et al. J Pharm Biomed Anal, 2016, 117:544
[12] Qi Y, Li S Z, Pi Z F, et al. J Chromatogr B, 2014, 953/954:11
[13] Sun W Y, Sun J N, Liu Z Q, et al. Chinese Journal of Pharmaceutical Sciences, 2005, 40(9):678 孙文燕, 孙建宁, 刘振权, 等. 中国药学杂志, 2005, 40(9):678
[14] Yue R C, Zhao L, Hu Y H, et al. J Ethnopharmacol, 2013, 145(2):465
[15] Xu T F, Liu S, Zhao J D, et al. Mol Biosyst, 2015, 11(11):3081
[16] Yue R, Zhao L, Hu Y, et al. Evid Based Complement Alternat Med, 2013, 2013:439690
[17] Wang Z G, Tang Z Y, Wang F, et al. Chinese Journal of Practical Department of Internal Medicine, 1999, 19(4):205 王振刚, 唐志毅, 王芳, 等. 中国实用内科杂志, 1999, 19(4):205
[18] Yang S B, Li X, Su M M, et al. Journal of Shanghai University of Traditional Chinese Medicine, 2009, 23(1):33 杨松滨, 李昕, 苏明明, 等. 上海中医药大学学报, 2009, 23(1):33
[19] Kim S, Hwang J, Xuan J H, et al. PloS One, 2014, 9(6):97501
[20] Zhang C F, Zhang S L, He X, et al. J Ethnopharmacol, 2014, 153(3):793
[21] Lin Y, Cui D H, Shen B. Journal of Medical Research, 2014, 43(9):108 林艳, 崔栋华, 沈波. 医学研究杂志, 2014, 43(9):108
[22] Chen J, Tang Q, Xu J H. Anhui Medical Journal, 2011, 32(8):1083 陈军, 唐茜, 徐建华. 安徽医学, 2011, 32(8):1083