Determination of trace amounts of oxytetracycline in surface water samples by bubble enrichment-high performance liquid chromatography

doi:10.3724/SP.J.1123.2018.02003

Abstract

Abstract:

A method was established to determine the trace amounts of oxytetracycline (OTC) in surface water samples by bubble enrichment-high performance liquid chromatography (HPLC). A novel sample pretreatment method, bubble enrichment, was used to enrich trace amounts of oxytetracycline in aqueous solutions, and the effects of bubble enrichment conditions on the enrichment efficiency was investigated. Enrichment factor of oxytetracycline had a maximum of 37.06, RSD was 4.8% (n=11), and LOD was 0.038 mg/L under the optimized conditions of bubble enrichment and chromatography. The method was applied to the determination of oxytetracycline in surface water samples, with an average recovery of 101.9%. It is obvious that bubble enrichment has a positive effect on the enrichment of oxytetracycline, and hence, can be combined with chromatography to realize rapid, sensitive, and accurate detection of oxytetracycline in surface water samples. Additionally, the bubble enrichment method used in this paper does not require any organic solvent for the pretreatment of samples, and furthermore, the device is simple, inexpensive, and easy to operate; therefore, it is a very green sample pretreatment method with great research and promotion value. It is expected to be applicable to the analysis of other trace substances in complex samples.

Key words: bubble enrichment, high performance liquid chromatography(HPLC), oxytetracycline(OTC), surface water

CLC Number:

O658

XIONG Fangyong, CAI Yunfeng, ZHANG Ying, DING Jianhua. Determination of trace amounts of oxytetracycline in surface water samples by bubble enrichment-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2018, 36(7): 665-669.

References

[1] Psillakis E, Kalogerakis N. TrAC-Trends Anal Chem, 2003, 22:565
[2] Rasmussen K E, Pedersen-Bjergaard S. TrAC-Trends Anal Chem, 2004, 23:1
[3] Zhang Y, Ding J H, Xiang H L, et al. Journal of Instrumental Analysis, 2017, 36(11):1352 张英, 丁健桦, 向虹霖, 等. 分析测试学报, 2017, 36(11):1352
[4] Cao J P, Xie Q L, Zhou J M, et al. Journal of Instrumental Analysis, 2015, 34(5):616 曹江平, 解启龙, 周继梅, 等. 分析测试学报, 2015, 34(5):616
[5] Hu G D. Chinese Journal of Chromatography, 2009, 27(1):1 胡国栋. 色谱, 2009, 27(1):1
[6] Liu Q, Shi J, Sun J, et al. Angew Chem Int Ed, 2011, 50:5913
[7] Reimann O, Smet-Nocca C. Angew Chem Int Ed, 2015, 54:306
[8] Wan I W, Nodeh H R, Aboulenein H Y, et al. Crit Rev Anal Chem, 2015, 45:1
[9] Baek C, Min J. Biochip J, 2015, 9:332
[10] Ho T S, Halvorsen T G, Pedersen-Bjergaard S, et al. J Chromatogr A, 2003, 998:61
[11] Ho T S, Reubsaet J L E, Anthonsen H S, et al. J Chromatogr A, 2005, 1072:29
[12] Keene W C, Maring H, Maben J R, et al. J Geophys Res, 2007, 112:88
[13] Péron N, Cagna A, Valade M, et al. Adv Colloid Interfac, 2000, 88(1):19
[14] Peron N, Meunier J, Cagna A, et al. J Microsc, 2004, 214:89
[15] Schmittkopplin P, Ligerbelair G, Koch B P, et al. Biogeosciences, 2011, 9:1571
[16] Gantt B, Meskhidze N. Atmos Chem Phys, 2013, 12:3979
[17] Chingin K, Cai Y F, Chagovets V, et al. Metabolomics, 2016, 12:171
[18] Mcneill V F, Sareen N, Schwier A N. Top Curr Chem, 2013, 339:201
[19] Chingin K, Cai Y, Liang J, et al. Anal Chem, 2016, 88(10):5033
[20] Vignati E, Facchini M C, Rinaldi M, et al. Atmos Environ, 2010, 44(5):670
[21] Blanchard D C. Adv Chem, 2009, 10(22):360
[22] Chingin K, Cai Y F, Liang J H, et al. Anal Chem, 2016, 88(10):5033
[23] Chen G X, Wu Y B. Progress In Veterinary Medicine, 2011, 32(5):102 陈桂秀, 吴银宝. 动物医学进展, 2011, 32(5):102
[24] Liu J C, Lu G H, Yang X F, et al. The Administration and Technique of Environmental Monitoring, 2012, 24(4):14 刘建超, 陆光华, 杨晓凡, 等. 环境监测管理与技术, 2012, 24(4):14
[25] Li L Y, Xu X M, Lin H, et al. Chinese Journal of Chromatography, 2016, 34(9):835 李凌云, 许晓敏, 林桓, 等. 色谱, 2016, 34(9):835
[26] Lai G, Chen G, Chen T, et al. Food Anal Method, 2015, 8:2052
[27] Zhang A Z, Wang Q L, Cao L L, et al. Chinese Journal of Chromatography, 2016, 34(2):58 张爱芝, 王全林, 曹丽丽, 等. 色谱, 2016, 34(2):158
[28] Ding J H, Gu H W, Yang S P, et al. Anal Chem, 2009, 81(20):8632
[29] Qi Y J, Liu F. Rock and Mineral Analysis, 2014, 33(1):1 祁彦洁, 刘菲. 岩矿测试, 2014, 33(1):1