Simultaneous determination of nine mycotoxins in cereal and cereal products by high performance liquid chromatography with composite immunoaffinity clean-up column

doi:10.3724/SP.J.1123.2018.08008

Abstract

Abstract:

A rapid analytical method using high performance liquid chromatography (HPLC) with post-column derivatization was established for the simultaneous determination of nine mycotoxins in cereal and cereal products. The nine mycotoxins were extracted from the samples by using acetone-water (80:20, v/v). The extract was purified by a self-made composite immunoaffinity clean-up column and was analyzed by HPLC. Mycotoxins were detected by fluorescence detection after on-line photochemical derivatization and were quantified by an external standard method. The nine compounds showed good linearities in their concentration ranges with the correlation coefficients (R²) exceeding 0.999. The spiked recoveries in real samples for the nine mycotoxins were above 80%, while the relative standard deviations (RSDs) were in the range of 1.0%-5.6%. The limits of detection (LODs) and the limits of quantification (LOQs) were 0.02-5.00 and 0.07-16.70 μg/kg, respectively. The developed method is simple, sensitive, and has good repeatability, and thus can be used for the routine analysis of the nine mycotoxins in cereal and cereal products.

Key words: cereal and cereal products, composite immunoaffinity column, high performance liquid chromatography (HPLC), mycotoxin

CLC Number:

O658

WANG Weigang, QIANG Min, DUAN Liqin. Simultaneous determination of nine mycotoxins in cereal and cereal products by high performance liquid chromatography with composite immunoaffinity clean-up column[J]. Chinese Journal of Chromatography, 2018, 36(12): 1330-1336.

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